FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459881
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG30930.6725652940652038No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT28140.6118974256383717No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27370.5951539637427944No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT26570.5777581591759607No Hit
CATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAATTTTAT26470.5755836836051066No Hit
GGGTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAA24520.5331814099734496No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC23910.5199171089912391No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22930.4986072483968679No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC21770.4733833317749592No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT21380.46490287704862776No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18320.39836392458048925No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT17750.38596941382662037No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG16990.36944339948812843No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC15860.344871825537476No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGCGGCAATGAT15140.32921560142732575No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG14750.3207351467009944No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA14550.316386195559286No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT14040.3052963701479296No Hit
ATCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCAT13690.2976857056499399No Hit
GATCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCA13370.2907273838232064No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG13090.28463885222481466No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA13060.2839865095535584No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC13040.2835516144393876No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA12550.272896684142202No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12550.272896684142202No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT12290.26724304765798107No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC12210.2655034672012977No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG12120.2635464391875289No Hit
GTTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCAAAGACTGT12030.26158941117376017No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC11760.2557183271324538No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC11730.25506598446119755No Hit
GAATAACCCCATCGCTTTGTTTTGTTAGGGTTTAAATGTATACCCAGAGA11610.25245661377617257No Hit
GATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC11610.25245661377617257No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC11510.25028213820531836No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT11290.2454982919494391No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT11090.2411493408077307No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGATCAT10880.2365829421089369No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10860.23614804699476602No Hit
GTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAAT10730.2333212287526556No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCC10700.23266888608139935No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10260.22310119356964087No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG10160.22092671799878666No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9890.2150556339574803No Hit
TTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCAAAGACTGTT9770.21244626327245525No Hit
CAGTAAACCCTGTTCCGACCACTGCCTCTCACTTATCGTCAATCTTCTCG9700.21092413037285732No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC9500.20657517923114893No Hit
GGATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC9380.2039658085461239No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG9200.20005175251858634No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC9100.19787727694773213No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9080.19744238183356128No Hit
GGATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC8990.19548535381979248No Hit
GGTTATGTCATTGGATGTTATGGATCATTGCCGCAAGAACACATCATACA8910.19374577336310914No Hit
GTCTCTGGGTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATT8760.19048406000682785No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8740.190049164892657No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAA8730.18983171733557158No Hit
ACCCTGTTCCGACCACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATT8630.1876572417647174No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT8600.18700489909346113No Hit
GACATAACCCATAAAATTTAGAGAATAACCCCATCGCTTTGTTTTGTTAG8590.18678745153637571No Hit
GAACATGGAGAACATTACATCAGGATTCCTAGGACCCCTTCTCGTGTTAC8560.18613510886511944No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAA8460.18396063329426526No Hit
TAACAAAACAAAGCGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCA8450.18374318573717985No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC8140.17700231146753181No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8040.17482783589667764No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT8020.17439294078250678No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7980.1735231505541651No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT7930.172435912768738No Hit
ATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCGCT7810.16982654208371298No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGCAGC7780.1691741994124567No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGG7770.1689567518553713No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC7750.16852185674120043No Hit
GTCTTTGATTTTTTGTATGATGTGTTCTTGCGGCAATGATCCATAACATC7730.1680869616270296No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT7720.1678695140699442No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC7550.16417290559949205No Hit
GTTTTGTTAGGGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAAC7390.16069374468612532No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7260.15786692644401487No Hit
ACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTT7250.15764947888692946No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACCACTGCCTCTCACTTATCGTCA7130.1550401082019044No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGAC6860.14916902416059807No Hit
TTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6860.14916902416059807No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6850.14895157660351266No Hit
AAACAAAGCGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGA6800.14786433881808556No Hit
CATTGGATGTTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCA6740.14655965347557304No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6710.14590731080431676No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6550.14242814989095004No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6540.14221070233386462No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6530.1419932547767792No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6520.1417758072196938No Hit
GCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6510.14155835966260835No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA6370.13851409386341249No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6330.1376443036350708No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC6300.13699196096381455No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6300.13699196096381455No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT6270.13633961829255828No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC6150.13373024760753324No Hit
TTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAATTTTATGG6090.13242556226502072No Hit
ATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAA5960.12959874402291027No Hit
GGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAA5950.12938129646582486No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT5870.12764171600914148No Hit
ATGTCATTGGATGTTATGGATCATTGCCGCAAGAACACATCATACAAAAA5850.12720682089497065No Hit
TCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTG5750.12503234532411647No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5740.12481489776703103No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT5730.12459745020994561No Hit
GATGTGTTCTTGCGGCAATGATCCATAACATCCAATGACATAACCCATAA5680.12351021242451851No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5680.12351021242451851No Hit
CATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCGCTTTGT5680.12351021242451851No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5600.12177063196783515No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5520.12003105151115179No Hit
GCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG5520.12003105151115179No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCT5500.11959615639698096No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5500.11959615639698096No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTT5490.11937870883989554No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGAT5490.11937870883989554No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5460.11872636616863928No Hit
CCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG5420.1178565759402976No Hit
GCCTCTCACTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA5370.1167693381548705No Hit
ATGTGTTCTTGCGGCAATGATCCATAACATCCAATGACATAACCCATAAA5350.11633444304069968No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5350.11633444304069968No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5350.11633444304069968No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA5280.11481231014110171No Hit
GTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAAC5280.11481231014110171No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5270.1145948625840163No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5260.11437741502693088No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5210.11329017724150377No Hit
CCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCG5190.11285528212733294No Hit
CTGATGGCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACA5180.11263783457024752No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA5130.11155059678482043No Hit
ATGTTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCAAAGACT5100.11089825411356416No Hit
TTTTATGGGTTATGTCATTGGATGTTATGGATCATTGCCGCAAGAACACA5090.11068080655647874No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5080.11046335899939333No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5070.1102459114423079No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG5070.1102459114423079No Hit
CTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGATCATTGCCGCAA5060.11002846388522249No Hit
ATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTTGCTGCACCT5040.10959356877105166No Hit
CATCCAACGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGAT5040.10959356877105166No Hit
GGTTATCGTTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5010.10894122609979538No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4990.10850633098562455No Hit
GGATGTTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCAAAGA4980.10828888342853911No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG4910.10676675052894119No Hit
CCCATAAAATTTAGAGAATAACCCCATCGCTTTGTTTTGTTAGGGTTTAA4900.10654930297185577No Hit
GTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC4860.10567951274351409No Hit
CCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGT4840.10524461762934324No Hit
AATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAC4820.10480972251517241No Hit
GCATGGTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGG4770.10372248472974531No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT4770.10372248472974531No Hit
CCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG4720.10263524694431819No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG4700.10220035183014736No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT4700.10220035183014736No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG4700.10220035183014736No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4670.10154800915889109No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT4660.10133056160180567No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAT4650.10111311404472026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)