FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459881
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG35860.7797669397083158No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT27800.6045042086974673No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT27670.601677390455357No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27340.5945016210715381No Hit
CATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAATTTTAT27090.5890654321444025No Hit
GGGTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAA24090.5238311650187766No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC23960.5210043467766662No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23060.5014340666389784No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT22920.49838980083978246No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC22010.47860207314500924No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG18930.41162822556269996No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT18150.39466731611003714No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA17890.38901367962581623No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA16180.3518301473642094No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGCGGCAATGAT16080.34965567179335527No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT15340.33356455256903417No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC14860.323127069828934No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC14400.3131244822030047No Hit
ATCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCAT14260.3100802164038088No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG14080.30616616037627126No Hit
GATCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCA13010.2828992717681313No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA12870.2798550059689354No Hit
GTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAAT12520.2722443414709457No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG12330.2681128378863228No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT12270.26680815254381024No Hit
GTTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCAAAGACTGT12260.2665907049867248No Hit
GAATAACCCCATCGCTTTGTTTTGTTAGGGTTTAAATGTATACCCAGAGA12160.2644162294158706No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC11720.25484853690411213No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC11660.25354385156159964No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11640.2531089564474288No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA11610.25245661377617257No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG11480.24962979553406206No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11400.24789021507737868No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT11350.2468029772919516No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGATCAT11300.2457157395065245No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11270.24506339683526826No Hit
GATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC10980.23875741767979108No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10960.23832252256562023No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC10900.23701783722310774No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCC10610.23071185806763056No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC10430.226797802040093No Hit
CAGTAAACCCTGTTCCGACCACTGCCTCTCACTTATCGTCAATCTTCTCG10260.22310119356964087No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAA9970.21679521441416363No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9950.2163603192999928No Hit
GTCTCTGGGTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATT9900.21527308151456573No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG9850.21418584372913863No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT9710.21114157792994276No Hit
GGATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC9460.20570538900280727No Hit
TTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCAAAGACTGTT9380.2039658085461239No Hit
GGTTATGTCATTGGATGTTATGGATCATTGCCGCAAGAACACATCATACA9300.20222622808944052No Hit
ACCCTGTTCCGACCACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATT9250.20113899030401342No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG9170.19939940984733004No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC9170.19939940984733004No Hit
GACATAACCCATAAAATTTAGAGAATAACCCCATCGCTTTGTTTTGTTAG8990.19548535381979248No Hit
GTCTTTGATTTTTTGTATGATGTGTTCTTGCGGCAATGATCCATAACATC8880.1930934306918529No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8760.19048406000682785No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC8760.19048406000682785No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8690.18896192710722992No Hit
TAACAAAACAAAGCGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCA8560.18613510886511944No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT8480.1843955284084361No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACCACTGCCTCTCACTTATCGTCA8440.18352573818009443No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAA8370.18200360528049647No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8300.18048147238089854No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC8260.17961168215255688No Hit
GGATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC8100.17613252123919013No Hit
GTTTTGTTAGGGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAAC7990.17374059811125053No Hit
GAACATGGAGAACATTACATCAGGATTCCTAGGACCCCTTCTCGTGTTAC7870.1711312274262255No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7780.1691741994124567No Hit
ATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCGCT7690.1672171713986879No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA7450.16199843002863784No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT7300.15873671667235653No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGG7220.15699713621567318No Hit
TTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC7110.15460521308773356No Hit
GCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7100.15438776553064815No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC7040.15308308018813563No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7030.1528656326310502No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGCAGC7030.1528656326310502No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA6970.1515609472885377No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT6940.15090860461728142No Hit
AAACAAAGCGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGA6900.15003881438893973No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6860.14916902416059807No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6790.14764689126100014No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6760.14699454858974387No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC6760.14699454858974387No Hit
ACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTT6670.14503752057597508No Hit
ATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAA6660.14482007301888966No Hit
CATTGGATGTTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCA6600.14351538767637717No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6600.14351538767637717No Hit
GGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAA6590.14329794011929173No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC6560.14264559744803548No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6510.14155835966260835No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6470.1406885694342667No Hit
ATGTCATTGGATGTTATGGATCATTGCCGCAAGAACACATCATACAAAAA6370.13851409386341249No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6190.13460003783587493No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6140.13351280005044783No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6120.133077904936277No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6080.1322081147079353No Hit
TTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAATTTTATGG6040.13133832447959362No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA6020.1309034293654228No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6010.13068598180833738No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCT5980.13003363913708113No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGAC5940.12916384890873944No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC5930.12894640135165403No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT5840.12698937333788524No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT5820.1265544782237144No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGAT5780.12568468799537272No Hit
GCCTCTCACTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA5770.1254672404382873No Hit
CATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCGCTTTGT5760.1252497928812019No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTT5680.12351021242451851No Hit
CTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGATCATTGCCGCAA5620.12220552708200601No Hit
GCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG5620.12220552708200601No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5600.12177063196783515No Hit
GGATGTTATGGATCATTGCCGCAAGAACACATCATACAAAAAATCAAAGA5550.12068339418240806No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA5530.12024849906823723No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5510.11981360395406637No Hit
TTTTATGGGTTATGTCATTGGATGTTATGGATCATTGCCGCAAGAACACA5380.11698678571195592No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA5370.1167693381548705No Hit
GATGTGTTCTTGCGGCAATGATCCATAACATCCAATGACATAACCCATAA5350.11633444304069968No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT5280.11481231014110171No Hit
GTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAAC5280.11481231014110171No Hit
GGTTATCGTTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5230.11372507235567463No Hit
CCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG5220.11350762479858921No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5210.11329017724150377No Hit
CCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGT5210.11329017724150377No Hit
CCCATAAAATTTAGAGAATAACCCCATCGCTTTGTTTTGTTAGGGTTTAA5200.11307272968441835No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG5200.11307272968441835No Hit
AATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAC5180.11263783457024752No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5090.11068080655647874No Hit
CCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5090.11068080655647874No Hit
GTGTTCTTGCGGCAATGATCCATAACATCCAATGACATAACCCATAAAAT5050.10981101632813707No Hit
GTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG5050.10981101632813707No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5040.10959356877105166No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC5030.10937612121396621No Hit
ATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTTGCTGCACCT4920.1069841980860266No Hit
CCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCG4910.10676675052894119No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG4910.10676675052894119No Hit
TCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTG4890.10633185541477033No Hit
GGTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAA4880.10611440785768492No Hit
GGGTTATGTCATTGGATGTTATGGATCATTGCCGCAAGAACACATCATAC4880.10611440785768492No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG4870.1058969603005995No Hit
ACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCG4850.10546206518642866No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA4800.10437482740100155No Hit
GTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC4780.10393993228683072No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT4750.10328758961557447No Hit
GTATGATGTGTTCTTGCGGCAATGATCCATAACATCCAATGACATAACCC4720.10263524694431819No Hit
GTTCTTGCGGCAATGATCCATAACATCCAATGACATAACCCATAAAATTT4710.10241779938723278No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4700.10220035183014736No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG4690.10198290427306195No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT4680.10176545671597653No Hit
GCAATGATCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAA4680.10176545671597653No Hit
GTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG4670.10154800915889109No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4650.10111311404472026No Hit
GCGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATG4620.100460771373464No Hit
GTTAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTT4610.10024332381637859No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGTTGGATGTGTC4600.10002587625929317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)