FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498892
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28790.5770788066355043No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14210.2848311859079721No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA13670.27400719995510053No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13380.26819431861003984No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13190.26438587910810357No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13170.2639849907394787No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13090.2623814372649792No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT13020.26097832797479215No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC12340.24734812344154644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12220.24494279322979726No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11750.23552191656711274No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11280.2261010399044282No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10270.20585617728887212No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10040.20124596104968612No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9980.20004329594381148No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCCGTACG9970.19984285175949904No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATT9390.18821708906937776No Hit
CAATAACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG9090.18220376354000464No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8920.17879621240669322No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8740.17518821708906937No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCGAGATATTGGATCTTC8680.17398555198319476No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8630.17298333106163258No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT8520.17077844503419576No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8450.1693753357440087No Hit
CATTTGAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT8200.1643642311361978No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAA8090.162159345108761No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAAAAGTCAGAAGGCAAAAACGAGA8010.1605557916342615No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7970.1597540148970118No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCA7870.15774957305388743No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7760.1555446870264506No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7740.15514379865782574No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7610.15253802426176405No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7600.15233758007745163No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7580.15193669170882676No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCTGTAATGTCAACGACCGACCT7430.1489300289441402No Hit
ATCCAATATCTCGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC7350.14732647546964073No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7250.14532203362651636No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT7220.14472070107357907No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7090.14211492667751738No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA6940.13910826391283082No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA6920.13870737554420595No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6880.13790559880695621No Hit
GAATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA6720.13469849185795724No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6710.13449804767364482No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6670.13369627093639505No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6670.13369627093639505No Hit
GTACAAAGACCTTTAGTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6670.13369627093639505No Hit
GGACATCCTTTGTTTACGTCCCGTCGACGCTGAATCCTGCGGACGACCCT6620.13269405001483286No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6560.13149138490895826No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTGGGCGA6540.1310904965403334No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6460.1294869430658339No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6420.12868516632858415No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6320.12668072448545978No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6300.1262798361168349No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6300.1262798361168349No Hit
GTGTAAATGTATCTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6200.12427539427371055No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGAACAACAGAGT6080.1218700640619613No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6080.1218700640619613No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6070.12166961987764888No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6020.12066739895608669No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC5910.1184625129286499No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5890.11806162456002502No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5850.11725984782277526No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5840.11705940363846284No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA5830.1168589594541504No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5750.1152554059796509No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5740.11505496179533847No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5640.1130505199522141No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT5620.11264963158358925No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5620.11264963158358925No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5580.1118478548463395No Hit
ATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGT5490.11004385718752757No Hit
ACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAA5460.10944252463459024No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC5460.10944252463459024No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5450.10924208045027782No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG5440.10904163626596537No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5430.10884119208165294No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5400.10823985952871565No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA5360.1074380827914659No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5310.10643586186990371No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5260.10543364094834151No Hit
GAACAACAGAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCGGCTGCGA5180.10383008747384204No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAGATACATTTACACC5130.10282786655227984No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT5120.10262742236796742No Hit
GATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCC5110.10242697818365498No Hit
CTCTTTACATTTTATGGGCTATGTTATTGGATGTTATGGGTCATTGCCAC5060.1014247572620928No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC5050.10122431307778036No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5040.10102386889346791No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5020.10062298052484306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)