FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498892
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA26950.540197076722016No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA15230.3052764927078406No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14570.29204717654321977No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13570.27200275811197616No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13560.2718023139276637No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12830.2571698884728558No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC12440.24935256528467084No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12270.2459450141513594No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12220.24494279322979726No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12150.2435396839396102No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11960.2397312444376739No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11860.23772680259454954No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11790.23632369330436245No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11600.2325152538024262No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCGAGATATTGGATCTTC9900.19843974246931198No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCCGTACG9660.19362908204581353No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC9460.1896201983595648No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8650.17338421943025745No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8630.17298333106163258No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8590.17218155432438284No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATT8540.17117933340282065No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8500.17037755666557092No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT8450.1693753357440087No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC8390.16817267063813413No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8230.16496556368913512No Hit
GGACATCCTTTGTTTACGTCCCGTCGACGCTGAATCCTGCGGACGACCCT7990.16015490326563667No Hit
CAATAACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG7770.15574513121076305No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7710.15454246610488845No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7700.154342021920576No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7690.15414157773626355No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7380.14792780802257804No Hit
CATTTGAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT7360.14752691965395315No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAA7320.1467251429167034No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCTGTAATGTCAACGACCGACCT7200.14431981270495417No Hit
ATCCAATATCTCGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC7010.14051137320301788No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7000.14031092901870545No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTGGGCGA6880.13790559880695621No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6850.1373042662540189No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA6740.1350993802265821No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6710.13449804767364482No Hit
GTGTAAATGTATCTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6670.13369627093639505No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6600.132293161646208No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAAAAGTCAGAAGGCAAAAACGAGA6600.132293161646208No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6480.12988783143445876No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT6280.12587894774821No Hit
CTCTTTACATTTTATGGGCTATGTTATTGGATGTTATGGGTCATTGCCAC6240.1250771710109603No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA6140.12307272916783592No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6140.12307272916783592No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCA6120.12267184079921105No Hit
GTACAAAGACCTTTAGTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6080.1218700640619613No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6020.12066739895608669No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACATCCTTTGTTTA6010.12046695477177424No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6000.12026651058746182No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5980.11986562221883695No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5800.1162576269012131No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5780.11585673853258822No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG5750.1152554059796509No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5720.11465407342671359No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGAACAACAGAGT5700.11425318505808872No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5670.11365185250515142No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5660.11345140832083897No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5650.11325096413652655No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5620.11264963158358925No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG5600.11224874321496436No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5600.11224874321496436No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5600.11224874321496436No Hit
ATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGT5530.11084563392477732No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5530.11084563392477732No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5400.10823985952871565No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG5390.1080394153444032No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5350.10723763860715345No Hit
GAATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5340.10703719442284101No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5310.10643586186990371No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC5300.10623541768559126No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5300.10623541768559126No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC5290.10603497350127884No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5190.10403053165815447No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5180.10383008747384204No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC5090.10202608981503011No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT5050.10122431307778036No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5050.10122431307778036No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5040.10102386889346791No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5040.10102386889346791No Hit
GTTTACGTCCCGTCGACGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG4990.10002164797190574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)