FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432639
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA36470.8429660756427414No Hit
CAATAACATAACCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG18950.43800951832821355No Hit
CTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT17940.41466441998987613No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT16630.3843851340262898No Hit
CTCTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14410.333072145599449No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA13930.32197744539905093No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGGAT12490.28869334479785685No Hit
CTCTATGGAAGGCGGGCATATTATATAAGAGAGAAACAACACATAGCGCC11090.25633380254669597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10820.25009303368397207No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT10630.24570138152131454No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10450.24154086894616528No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10340.23899833348357408No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT10330.23876719389606577No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9930.22952161039573407No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9890.2285970520457009No Hit
CTTTTTACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAAA9840.22744135410815947No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9780.2260545165831097No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9350.21611551432025317No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA9170.2119550017451039No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC8600.19878004525713125No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA8340.19277041598191563No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8300.19184585763188247No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8170.18884104299427468No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8160.1886099034067664No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA8160.1886099034067664No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT8130.18791648464424152No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7870.1819068553690259No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7780.17982659908145127No Hit
CTCTATGACCGTTTCTCTTCCGAAAGTGAGACAAGAAATGTGAAACCACA7390.17081215516862788No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7360.170118736406103No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT7310.16896303846856156No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7190.16618936341846205No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7150.1652648050684289No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC7090.16387796754337913No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7090.16387796754337913No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6960.1608731529057713No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6930.16017973414324643No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6840.15809947785567183No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6690.15463238404304744No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6270.14492452136769918No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC6220.1437688234301577No Hit
ATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6130.14168856714258307No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5910.13660349621740064No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5900.13637235662989236No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5890.13614121704238405No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACG5830.1347543795173343No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG5830.1347543795173343No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5820.13452323992982604No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5790.13382982116730116No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5750.13290526281726797No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5740.1326741232297597No Hit
GTGTTGTTTCTCTCTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAA5740.1326741232297597No Hit
CCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT5660.13082500652969334No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAA5650.13059386694218506No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC5630.13013158776716846No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGG5610.1296693085921519No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5590.1292070294171353No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5570.12874475024211873No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC5550.12828247106710214No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5530.12782019189208554No Hit
TTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACCAGACATTATT5520.12758905230457726No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5490.12689563354205238No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5360.12389081890444459No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA5160.11926802715427874No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5140.11880574797926216No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5080.1174189104542124No Hit
TCCTCAGGCCATGCAATGGAACTCCACAACCTTCCACCAAACTCTGCAAG5060.11695663127919582No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA4940.11418295622909631No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAAAGGGGTAGGCTGCCT4930.11395181664158802No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4910.11348953746657144No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4880.11279611870404656No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACG4860.11233383952902998No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4840.11187156035401338No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4830.1116404207665051No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4740.10956016447893047No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4710.10886674571640559No Hit
TTGTAGGCCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATG4680.10817332695388071No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4650.10747990819135583No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC4630.10701762901633927No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4610.10655534984132269No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATGTAG4590.10609307066630608No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC4570.10563079149128951No Hit
CTTTGTACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAAA4450.10285711644119No Hit
ATGATGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATACAGAG4420.10216369767866512No Hit
GTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACA4400.10170141850364854No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4350.10054572056610708No Hit
CAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG4340.10031458097859879No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4330.1000834413910905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)