FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432639
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA37200.8598392655308467No Hit
CAATAACATAACCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG18440.42622139936529074No Hit
CTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT17430.4028763010269532No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15250.3524878709501455No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGGAT14490.3349212622995153No Hit
CTCTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14100.3259068183866919No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA13940.32220858498655924No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11420.2639614089344696No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT10920.252404429559055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10830.2503241732714804No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10780.24916847533393893No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10620.24547024193380626No Hit
CTCTATGGAAGGCGGGCATATTATATAAGAGAGAAACAACACATAGCGCC10540.2436211252337399No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10280.23761149595852432No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10030.23183300627081702No Hit
CTTTTTACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAAA9990.23090844792078383No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC9540.2205071664829107No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA9460.21865804978284434No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT8860.20478967453234684No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8860.20478967453234684No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA8700.20109144113221417No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8180.18907218258178296No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA8020.1853739491816503No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8010.185142809594142No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT7990.1846805304191254No Hit
CTCTATGACCGTTTCTCTTCCGAAAGTGAGACAAGAAATGTGAAACCACA7420.17150557393115276No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7380.1705810155811196No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7030.16249113001832938No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7000.1617977112558045No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT6910.15971745496822987No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6890.15925517579321327No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6640.153476686105506No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6620.15301440693048937No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6580.15208984858045624No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6390.14769819641779866No Hit
ATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6350.1467736380677655No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6310.14584907971773234No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6200.1433065442551411No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6140.14191970673009136No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC6140.14191970673009136No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5980.13822147332995868No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5900.13637235662989236No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACG5890.13614121704238405No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5800.13406096075480942No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC5750.13290526281726797No Hit
GTGTTGTTTCTCTCTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAA5730.1324429836422514No Hit
TTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACCAGACATTATT5670.13105614611720165No Hit
CCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT5670.13105614611720165No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGG5650.13059386694218506No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5650.13059386694218506No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5630.13013158776716846No Hit
TCCTCAGGCCATGCAATGGAACTCCACAACCTTCCACCAAACTCTGCAAG5520.12758905230457726No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5500.12712677312956067No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5490.12689563354205238No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5410.12504651684198603No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG5380.12435309807946116No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5360.12389081890444459No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5210.1204237250918202No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5160.11926802715427874No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5100.11788118962922899No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAA5060.11695663127919582No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC4990.11533865416663777No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4960.1146452354041129No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC4890.11302725829155486No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4860.11233383952902998No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA4850.11210269994152168No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA4840.11187156035401338No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4840.11187156035401338No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4840.11187156035401338No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC4780.11048472282896366No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4750.10979130406643878No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATGTAG4740.10956016447893047No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4680.10817332695388071No Hit
ATCCAGAACATGTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC4610.10655534984132269No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAAAGGGGTAGGCTGCCT4580.10586193107879781No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACG4540.10493737272876463No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4470.1033193956162066No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4470.1033193956162066No Hit
CTTTGTACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAAA4460.1030882560286983No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4430.10239483726617342No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4410.10193255809115684No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT4360.10077686015361537No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4360.10077686015361537No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4350.10054572056610708No Hit
CAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG4340.10031458097859879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)