FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002490394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487455
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT34300.7036546963309434No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC29400.6031325968550943No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT28720.5891825912135479No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT26060.534613451498087No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC23770.4876347560287616No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23190.4757362218050897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23150.47491563323793995No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT22690.4654788647157173No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG21800.44722076909663455No Hit
ACAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT18710.3838303022843134No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC18230.37398323947851597No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18040.3700854437845545No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT16980.3483398467550851No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT16920.3471089639043604No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG15770.323517042598804No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15730.3226964540316542No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC15540.3187986583376927No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT15530.3185935111959053No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC14920.306079535546871No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA14850.3046435055543589No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAC14720.30197659271112204No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC14590.29930967986788526No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT14430.2960273255992861No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA14170.29069349991281246No Hit
CTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC13960.28638540993527606No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA13130.26935819716691795No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA12540.2572545158014586No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11820.2424839215927624No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT11770.24145818588382517No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG11750.24104789160025028No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT11740.24084274445846285No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG11380.23345744735411475No Hit
ATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTT11110.22791847452585368No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC11000.22566185596619176No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC10970.2250464145408294No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA10930.22422582597367963No Hit
GGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT10830.22217435455580514No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCA10740.22032803027971812No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10720.21991773599614323No Hit
CAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG10220.2096603789067709No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA9870.20248022894421022No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTAC9760.2002236103845483No Hit
GATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGT9740.19981331610097341No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9610.1971464032577366No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA9600.19694125611594915No Hit
GACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAG9520.19530007898164958No Hit
GTCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC9460.1940691961309249No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG9460.1940691961309249No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACA9380.19242801899662532No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC9330.1914022832876881No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG9250.1897611061533885No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC9130.18729934045193916No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG9090.18647875188478935No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8870.18196551476546552No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG8820.18093977905652828No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC8770.17991404334759106No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC8760.1797088962058036No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA8760.1797088962058036No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCT8550.17540080622826723No Hit
GGCCTATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCTTTTGGGTTTT8470.17375962909396767No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA8350.17129786339251826No Hit
GCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTA8270.16965668625821873No Hit
CATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGT8230.16883609769106891No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC8180.1678103619821317No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG8150.16719492055676932No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA8060.1653485962806823No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7940.16288683057923295No Hit
TTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTATGG7910.16227138915387063No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC7890.16186109487029574No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7820.1604250648777836No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC7720.15837359345990912No Hit
CCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGT7520.15427065062416018No Hit
CAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT7490.15365520919879783No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC7470.15324491491522294No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG7440.1526294734898606No Hit
ACAAAAAGGTGGGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATG7390.15160373778092337No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7390.15160373778092337No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7250.1487316777958991No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7240.14852653065411167No Hit
ACCCAAAAGACCCACAATACGCTGACAGACTTTCCAATCAATAGGCCTGT7210.14791108922874932No Hit
ACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA7190.14750079494517443No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGG7160.14688535351981208No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA7110.14585961781087486No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT7060.14483388210193762No Hit
TCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTT7020.14401329353478784No Hit
ACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG6990.1433978521094255No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA6990.1433978521094255No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACAT6960.14278241068406314No Hit
ATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT6960.14278241068406314No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6900.14155152783333846No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCA6860.14073093926618868No Hit
CAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAA6840.1403206449826138No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6760.1386794678483142No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCT6740.1382691735647393No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG6670.13683314357222717No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA6650.13642284928865228No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC6540.13416623072899037No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6440.13211475931111588No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT6420.131704465027541No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA6400.1312941707439661No Hit
CCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACC6390.13108902360217867No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC6350.13026843503502888No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6300.12924269932609164No Hit
GTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC6200.12719122790821716No Hit
AATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATA6170.12657578648285484No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6130.12575519791570502No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6000.12308828507246823No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT5960.12226769650531844No Hit
TTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA5870.12042137222923142No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5830.11960078366208163No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5730.11754931224420716No Hit
GTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAG5680.11652357653526993No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTT5670.11631842939348247No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5590.1146772522591829No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5550.11385666369203311No Hit
ACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCGAGGATT5550.11385666369203311No Hit
TGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACC5540.11365151655024566No Hit
TTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC5540.11365151655024566No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA5500.11283092798309588No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5490.11262578084130843No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5470.11221548655773353No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5410.11098460370700886No Hit
TTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACAT5310.10893313228913439No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5280.10831769086377203No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5250.1077022494384097No Hit
TTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACAT5180.10626621944589756No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT5160.10585592516232267No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5130.10524048373696034No Hit
TTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATC5110.10483018945338544No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5090.10441989516981054No Hit
ATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAAATGTA5070.10400960088623565No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC4980.10216327661014862No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACCGCA4940.10134268804299884No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4880.10011180519227415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)