FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002490395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487455
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT33320.6835502764357735No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC30100.6174928967802156No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT25670.5266127129683765No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC24940.5116369716178929No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT24420.5009693202449457No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG23650.4851729903273123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22930.4704023961186161No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20730.42527002492537774No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT20580.422192817798566No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC20510.4207567878060539No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18950.38875383368721217No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT17370.35634058528479556No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT16240.333158958262814No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC15960.3274148382927655No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC14800.30361776984542166No Hit
ACAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT14760.3027971812782718No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT14750.3025920341364844No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA13950.2861802627934886No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA13660.28023099568165266No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT13240.27161481572657986No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13210.27099937430121757No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG12060.24740745299566114No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA12060.24740745299566114No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT11690.2398170087495256No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT11640.23879127304058836No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC11330.2324317116451775No Hit
GGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT11150.22873906309300346No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG11130.22832876880942857No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA11060.22689273881691643No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAC11060.22689273881691643No Hit
CTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC10850.22258464883938003No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTAC10530.21601994030218175No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA10320.21171185032464535No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA10050.20617287749638427No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG9930.20371111179493495No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACA9910.20330081751136No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC9890.2028905232277851No Hit
GTCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC9850.20206993466063533No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9570.19632581469058685No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA9510.19509493183986215No Hit
ATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTT9460.1940691961309249No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC9350.191812577571263No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA9280.19037654757875086No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT9180.18832507616087638No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC9180.18832507616087638No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG9030.1852478690340647No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA9020.18504272189227725No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCA8920.18299125047440276No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8920.18299125047440276No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8870.18196551476546552No Hit
CAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG8870.18196551476546552No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG8810.18073463191474085No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC8620.17683683622077934No Hit
GATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGT8610.1766316890789919No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8510.17458021766111742No Hit
GACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAG8430.17293904052681786No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA8420.1727338933850304No Hit
GGCCTATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCTTTTGGGTTTT8400.1723235991014555No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC8160.1674000676985568No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC8100.1661691848478321No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACAT8080.16575889056425722No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG8010.16432286057174508No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7990.1639125662881702No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7740.158783887743484No Hit
TTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTATGG7710.15816844631812169No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC7640.15673241632560955No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA7610.1561169749002472No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA7580.15550153347488485No Hit
GCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTA7540.15468094490773507No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7540.15468094490773507No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7260.14893682493768656No Hit
CATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGT7240.14852653065411167No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA7170.14709050066159954No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC7140.1464750592362372No Hit
ACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA7060.14483388210193762No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7040.14442358781836273No Hit
ACAAAAAGGTGGGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATG6880.14114123354976357No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT6840.1403206449826138No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6780.1390897621318891No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6760.1386794678483142No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG6690.13724343785580206No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT6660.13662799643043974No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6630.1360125550050774No Hit
CAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT6610.1356022607215025No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA6580.13498681929614015No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT6510.13355078930362801No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC6480.1329353478782657No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6350.13026843503502888No Hit
CCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGT6340.13006328789324142No Hit
TTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA6330.12985814075145397No Hit
TCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTT6330.12985814075145397No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6300.12924269932609164No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG6250.1282169636171544No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6210.12739637505000462No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCT6190.12698608076642973No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCT6100.1251397564903427No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCA6070.12452431506498036No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6040.12390887363961801No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5970.1224728436471059No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5810.11919048937850674No Hit
GTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC5790.11878019509493185No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5770.11836990081135694No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5740.1177544593859946No Hit
TTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC5720.11734416510241971No Hit
ACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG5700.11693387081884482No Hit
ATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT5670.11631842939348247No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5570.114266957975608No Hit
CAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAA5550.11385666369203311No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA5490.11262578084130843No Hit
ACCCAAAAGACCCACAATACGCTGACAGACTTTCCAATCAATAGGCCTGT5460.11201033941594608No Hit
AATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATA5460.11201033941594608No Hit
CCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACC5390.11057430942343395No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5370.11016401513985906No Hit
TTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATC5270.10811254372198459No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGG5260.10790739658019714No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT5130.10524048373696034No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5110.10483018945338544No Hit
TTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACAT5110.10483018945338544No Hit
GTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAG5090.10441989516981054No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5090.10441989516981054No Hit
TTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACAT5060.1038044537444482No Hit
TGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACC4980.10216327661014862No Hit
CCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAAT4950.10154783518478629No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC4920.10093239375942395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)