FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285646
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA20570.7201221091840949No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11170.39104345938679347No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10790.3777402799269025No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8850.30982404794745944No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8180.2863684420576518No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8140.28496810737766326No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8080.2828676053576805No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGACAGAGAGTCCAA7950.2783165176477178No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6650.232805640548091No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6250.2188022937482058No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6240.2184522100782087No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5910.20689944896830342No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5790.20269844492833786No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5680.19884752455836943No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGCATT5680.19884752455836943No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5600.19604685519839243No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAATGGTGGGCGTTC5530.19359626950841252No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5400.18904518179844984No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5370.18799493078845844No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5250.1837939267484929No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5210.18239359206850436No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5200.18204350839850725No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5090.17819258802853882No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4780.1673399942586278No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT4740.1659396595786393No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC4720.16523949223864504No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTAAG4650.16278890654866512No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4580.16033832085868524No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4580.16033832085868524No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG4550.15928806984869384No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4500.1575376514987082No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4500.1575376514987082No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4450.15578723314872256No Hit
CTGTCATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4420.15473698213873116No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4180.14633497405880005No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4170.1459848903888029No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT4150.14528472304880866No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT4150.14528472304880866No Hit
ATGTAAGACCTTGGGCAAGAAATGGTGGGCGTTCACGGTGGTTTCCATGC4100.143534304698823No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4090.1431842210288259No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4090.1431842210288259No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4090.1431842210288259No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTCATGTCAACGACCGACCT4000.14003346799885175No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3980.13933330065885746No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATCCGCACTCCTCCAGC3960.1386331333188632No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC3930.13758288230887183No Hit
CTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3910.13688271496887758No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3880.13583246395888615No Hit
CCTCTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3840.13443212927889767No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3830.13408204560890052No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3790.132681710928912No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3790.132681710928912No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA3770.13198154358891773No Hit
AGCTGGAGGAGTGCGGATCCACACTCCGAAAGACACCAAATACTCTATGA3730.13058120890892924No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGACCCGTGGTCGGACGAAACGG3600.12603012119896656No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3590.12568003752896942No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3540.12392961917898376No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3540.12392961917898376No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3530.12357953550898663No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3520.1232294518389895No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGAGAGAGAACAACAGAGT3520.1232294518389895No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC3510.12287936816899239No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3480.12182911715900101No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3450.12077886614900962No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG3430.12007869880901534No Hit
GCTTTAATGCCTTTGTATGCATGCATTCAATCTAAGCAGGCTTTCACTTT3410.1193785314690211No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3400.11902844779902397No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3390.11867836412902684No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3380.11832828045902971No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT3360.11762811311903545No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC3350.11727802944903833No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC3350.11727802944903833No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG3310.1158776947690498No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3280.11482744375905841No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3260.11412727641906416No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3260.11412727641906416No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA3250.11377719274906703No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3240.1134271090790699No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3200.11202677439908137No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT3150.11027635604909573No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3120.10922610503910435No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3110.10887602136910722No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA3090.10817585402911295No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA3090.10817585402911295No Hit
GAGTTATCTGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3080.10782577035911582No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3070.1074756866891187No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAG3040.10642543567912731No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA3010.10537518466913592No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2970.1039748499891474No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA2960.10362476631915027No Hit
ATGTAAGACCTTGGGCAAGAAATGGTGGGCGTTCACGGTGGTCTCCATGC2930.10257451530915888No Hit
CCTCAAGGTCGGTCGTTGACATGACAGAGAGTCCAAGAGTCCTCTTATGT2910.10187434796916463No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG2900.1015242642991675No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG2900.1015242642991675No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA2890.10117418062917038No Hit
ATCCAATATCCAGGGACCTAGTAGTTAGTTATGTTAACACTAATATGGGC2890.10117418062917038No Hit
ACCTAGTAGTTAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAA2880.10082409695917326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)