FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285646
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19690.6893147462243476No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12980.45440860365627384No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9940.3479831679771465No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9030.31612555400740777No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7960.2786666013177149No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7760.2716649279177723No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGACAGAGAGTCCAA7350.25731149744789006No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7210.25241032606793024No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6200.21705187539822018No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6150.21530145704823453No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5790.20269844492833786No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGCATT5730.2005979429083551No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5670.19849744088837235No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5670.19849744088837235No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5560.1946465205184039No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5470.19149576748842975No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5310.18589442876847567No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5220.1827436757385015No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5160.18064317371851873No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5100.17854267169853594No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5100.17854267169853594No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4950.173291416648579No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAATGGTGGGCGTTC4900.17154099829859337No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC4820.16874032893861635No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4680.16383915755865652No Hit
CTGTCATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4560.15963815351869098No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4510.15788773516870533No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4470.15648740048871682No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT4410.15438689846873402No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4380.15333664745874265No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA4370.1529865637887455No Hit
AGCTGGAGGAGTGCGGATCCACACTCCGAAAGACACCAAATACTCTATGA4370.1529865637887455No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT4330.151586229108757No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4320.15123614543875985No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTCATGTCAACGACCGACCT4280.14983581075877134No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4270.14948572708877422No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTAAG4260.14913564341877708No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4230.1480853924087857No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4030.14108371900884312No Hit
CCTCTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4020.140733635338846No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT3970.13898321698886032No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA3890.13618254762888332No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC3890.13618254762888332No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3890.13618254762888332No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3870.13548238028888904No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3850.13478221294889478No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3810.13338187826890627No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3800.13303179459890915No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3780.1323316272589149No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3760.1316314599189206No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3730.13058120890892924No Hit
GCTTTAATGCCTTTGTATGCATGCATTCAATCTAAGCAGGCTTTCACTTT3720.1302311252389321No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG3680.1288307905589436No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3670.12848070688894647No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3660.12813062321894933No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3650.1277805395489522No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3620.12673028853896082No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATCCGCACTCCTCCAGC3600.12603012119896656No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3590.12568003752896942No Hit
CTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3560.12462978651897805No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC3550.12427970284898092No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGAGAGAGAACAACAGAGT3550.12427970284898092No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG3540.12392961917898376No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3450.12077886614900962No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3450.12077886614900962No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3390.11867836412902684No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG3390.11867836412902684No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT3370.11797819678903258No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3330.11657786210904407No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3320.11622777843904693No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3290.11517752742905554No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3220.11272694173907565No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGTCCGACCACGGGTCGCACCT3220.11272694173907565No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3200.11202677439908137No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3180.11132660705908712No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3140.1099262723790986No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA3140.1099262723790986No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA3140.1099262723790986No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3130.10957618870910148No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG3090.10817585402911295No Hit
ATGTAAGACCTTGGGCAAGAAATGGTGGGCGTTCACGGTGGTTTCCATGC3080.10782577035911582No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA3050.10677551934912444No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3010.10537518466913592No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC3010.10537518466913592No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3000.1050251009991388No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG2990.10467501732914167No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC2980.10432493365914453No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT2970.1039748499891474No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA2940.10292459897915601No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA2880.10082409695917326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)