FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513106
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG19230.374776361999275No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT16540.32235054745023445No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT14490.2823977891507798No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14130.27538169501038773No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT11410.22237120594964782No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT10190.1985944424738748No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG10070.19625574442707744No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC9890.19274769735688144No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG9520.1855367117125896No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACC9400.18319801366579225No Hit
GTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTACCTGATCTGG9250.18027464110729557No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG9140.178130834564398No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT9120.17774105155659845No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC9090.17715637704489912No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8960.174622787494202No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG8570.1670220188421106No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTACCCCATGAA8410.16390375477971414No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8340.16253951425241567No Hit
GGATCATATTGTACACAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA8190.159616141693919No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC8190.159616141693919No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8150.15883657567831988No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC8110.15805700966272077No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8080.15747233515102144No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGCTATTCCCTAAACTTCAT8050.15688766063932208No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7560.14733797694823292No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7560.14733797694823292No Hit
GTATACAGGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT7520.1465584109326338No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7470.14558395341313493No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7220.14071166581564043No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT7100.13837296776884309No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCAAAACCAGACATTATT6810.1327211141557495No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGCTATTCCCTAA6700.1305773076128519No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6480.1262896945270568No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6200.12083273241786298No Hit
ATATTGTACACAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6180.12044294941006342No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTACCCC6040.1177144683554665No Hit
GGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAATGGAGCAG6000.11693490233986739No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5980.11654511933206783No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5870.11440131278917026No Hit
CTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTC5740.11186772323847315No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5660.1103085912072749No Hit
CCTGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5280.10290271405908331No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5200.10134358202788507No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5190.1011486905239853No Hit
GTACATGACCCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5190.1011486905239853No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCCTGTAT5170.10075890751618574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)