FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444768
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14190.31904273688754586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12800.2877904885243543No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA12200.2743003093747752No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11240.2527160227354486No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9350.2102219584142744No Hit
ACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCG9130.20527555939276204No Hit
CTGTAAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCTTGGTGT8660.19470825239225842No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8180.18391610907259515No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8060.18121807324267933No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7570.17020109360385638No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT7560.16997625728469673No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7290.16390567666738615No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7260.1632311677099072No Hit
GCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTG6920.15558673285847902No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA6820.15333836966688252No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6750.15176451543276492No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6740.1515396791136053No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6540.14704295273041226No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA6470.1454690984962947No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6450.1450194258579754No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6330.14232139002805957No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6290.14142204475142095No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6210.13962335419814376No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCCGTACG6090.1369253183682279No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6030.13557630045327002No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5970.1342272825383121No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5890.1324285919850349No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5870.1319789193467156No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5860.13175408302755592No Hit
TTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCC5780.12995539247427873No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5720.1286063745593208No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5580.1254586660910857No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5540.12455932081444708No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5530.12433448449528743No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5400.12141161234621195No Hit
ATATATGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG5330.11983775811209439No Hit
CTCATATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5220.11736455860133822No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5120.11511619540974172No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5110.11489135909058204No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5010.11264299589898553No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5000.1124181595798259No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4990.11219332326066624No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4920.11061946902654868No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT4890.10994496006906972No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4860.10927045111159076No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4750.1067972516008346No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4700.10567307000503633No Hit
GAAAAGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCCAGTG4630.10409921577091877No Hit
ACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATAT4610.10364954313259946No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4600.10342470681343982No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4600.10342470681343982No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4560.1025253615368012No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4540.1020756888984819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)