FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427163
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20160.47195098826443305No Hit
CCTCAGGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17070.3996132623846166No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA16350.3827578699466012No Hit
GTAAAGTACCCCAACATCCAATTACATATCCCATGAAATTAAGGGAGTAG13200.3090155280302835No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12520.2930965462832689No Hit
CCTCTAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12310.2881803901555144No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11730.2746024351360019No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11500.269218073662747No Hit
AGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA11340.26547243089874356No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10810.25306498924298215No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGTGAGTCCAA10490.24557370371497533No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT10240.23972113689621996No Hit
GTACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT9750.22825010593145942No Hit
AGCTAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA9410.2202906150579521No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9210.2156085616029478No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8690.20343522261993666No Hit
GTACTAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT8540.19992368252868342No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8300.19430521838267828No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8100.18962316492767398No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8060.1886867542366731No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC8030.18798444621842247No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCTG8010.18751624087292204No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7720.18072726336316583No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG7610.17815213396291346No Hit
GTACCTTACCGCAAGAACATATTGTACTAAAAATCAAGCAATGTTTTCGA7500.1755770045626611No Hit
TTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACAT7450.17440649119891002No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT7160.16761751368915379No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7120.16668110299815292No Hit
GTCTTACATAAGAGGACTCTTGGACTCACGGCAATGTCAACGACCGACCT7030.16457417894340098No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7010.16410597359790058No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6920.16199904954314864No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6900.1615308441976482No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6750.158019304106395No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT6700.1568487907426439No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6590.15427366134239154No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6530.15286904530589027No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6510.15240083996038983No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6480.1516985319421392No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6460.15123032659663876No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6400.14982571056013747No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6390.14959160788738723No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6360.1488892998691366No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6290.1472505811598851No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6260.14654827314163446No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6190.14490955443238296No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6070.14210032235938036No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6030.14116391166837952No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5950.1392910902863778No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5850.13695006355887565No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5780.13531134484962415No Hit
ATTCCTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAACCTCG5750.1346090368313735No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5650.13226801010387135No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAT5640.13203390743112114No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5550.1299269833763692No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5540.129692880703619No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5540.129692880703619No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5440.12735185397611684No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5430.12711775130336664No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5300.12407441655761384No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5260.12313800586661297No Hit
TTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACAT5260.12313800586661297No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5170.12103108181186105No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5160.12079697913911083No Hit
ATGTTAATATGGGCCTAAAAATAAGACAACTACTGTGGTTTCACATTTCC5140.12032877379361041No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5050.11822184973885846No Hit
GTAAGGTACCCCAACATCCAATTACATATCCCATGAAATTAAGGGAGTAG5040.11798774706610826No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5040.11798774706610826No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5000.1170513363751074No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4930.1154126176658559No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4860.11377389895660438No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4860.11377389895660438No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4850.11353979628385417No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4830.11307159093835374No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4820.11283748826560352No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4810.11260338559285332No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4750.11119876955635202No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4730.11073056421085159No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4690.10979415351985074No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4660.1090918455016001No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG4630.10838953748334944No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4600.1076872294650988No Hit
GTCGTTGACATTGCCGTGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4590.10745312679234859No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4580.10721902411959837No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4520.10581440808309708No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4500.10534620273759665No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4490.10511210006484645No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC4450.10417568937384558No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4440.10394158670109536No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4440.10394158670109536No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4420.10347338135559493No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4380.10253697066459407No Hit
CCCTTAATTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA4310.10089825195534258No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACC4290.10043004660984214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)