FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460940
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT29100.6313186097973705No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT28190.6115763439927105No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT22200.4816245064433549No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19970.43324510782314396No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT18320.39744869180370546No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA16730.36295396363951926No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT15250.3308456632099623No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT12410.2692324380613529No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT12360.2681476981819759No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC12070.2618562068815898No Hit
ATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGAT12060.26163925890571443No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT11900.25816809129170826No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA11000.2386427734629236No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC10590.2297479064520328No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC10170.2206360914652666No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG9940.21564628802013278No Hit
CCATAAAGTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGT9910.21499544409250665No Hit
GTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGG9560.2074022649368681No Hit
TTCCTAAGGTTACCAAATATTTTCCTATGGAAAAGGGAATTAAACCCTAT9460.2052327851781143No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG9390.2037141493469866No Hit
TAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATATTTTCCTATG9270.20111077363648197No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG9000.19525317828784658No Hit
GTCTTAAAATAATGATTAACCACGTTATCAGGATAATAGGGTTTAATTCC8960.19438538638434502No Hit
GGAAAATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTT8940.19395149043259427No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCAGGAAGGTCAATCACCTGGAT8700.18874473901158503No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTATGGGTCACCATATT8600.18657525925283117No Hit
CCTTAGGAAAAAATCTGGATGGCATAACTAATTTTAATCTTCTCAATTCA8530.18505662342170348No Hit
AATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTA8370.1815854558076973No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC8190.17768039224194038No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA7750.16813468130342343No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA7740.16791773332754806No Hit
ATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACAAATTGTTCA7710.1672668893999219No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG7700.1670499414240465No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT7650.1659652015446696No Hit
ACACAAATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAA7650.1659652015446696No Hit
CTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTGTTCCTACT7630.16553130559291881No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTATGGGTCACCATA7430.1611923460754111No Hit
ATATTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTGG7360.15967371024428342No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA7350.15945676226840805No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTC7340.15923981429253267No Hit
ACTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTT7290.15815507441315574No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG7250.15728728250965418No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA7230.15685338655790343No Hit
CATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCCCTCT7180.1557686466785265No Hit
CAATAGGGCGATTTACAGGAAGTTTACGAAAACAATCTTTGATTTTTTGT7180.1557686466785265No Hit
TTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTATG7010.15208053108864494No Hit
CCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCT6940.15056189525751723No Hit
GGCCTACAAATTGTTCACATTTGTGTATCAAATCTTGATGTAAATGAATG6530.14166702824662647No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT6460.14014839241549876No Hit
CTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTC6310.13689417277736798No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA6300.1366772248014926No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC6240.13537553694624027No Hit
CCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCA6230.1351585889703649No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC6100.1323382652839849No Hit
ATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTA6100.1323382652839849No Hit
GCATAACTAATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACA6080.13190436933223415No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA6020.13060268147698184No Hit
GTGGTTAACTATGTCAATACTAACATGGGCCTAAAAATTAGACAATTGCT5930.12865014969410335No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5930.12865014969410335No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC5850.12691456588710026No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA5850.12691456588710026No Hit
GTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTTAGGAAAAAAT5850.12691456588710026No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG5850.12691456588710026No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG5840.12669761791122489No Hit
TGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGATA5740.12452813815247105No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA5680.12322645029721872No Hit
CCTACAAATTGTTCACATTTGTGTATCAAATCTTGATGTAAATGAATGTC5580.12105697053846488No Hit
TAAATACACAGTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCT5550.12040612661083873No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT5540.12018917863496333No Hit
CTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGAC5530.11997223065908795No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCC5500.1193213867314618No Hit
CGTTATCAGGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTG5470.11867054280383564No Hit
TTTTTGTACAATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACAT5450.11823664685208486No Hit
CATTCATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCC5410.11736885494858333No Hit
CATTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCAT5390.11693495899683255No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC5380.11671801102095718No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT5310.11519937518982948No Hit
GGTTAACTCCTTCTTTTCCTGACATTCATTTACATCAAGATTTGATACAC5290.11476547923807871No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTGGCAGCAGGATCTTCC5280.11454853126220332No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT5250.11389768733457717No Hit
GTCCGGCAGATGAGAAGGAACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5180.11237905150344948No Hit
ACCAAATATTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAA5120.11107736364819715No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT5100.1106434676964464No Hit
ATTTACAGGAAGTTTACGAAAACAATCTTTGATTTTTTGTACAATGTGAT5080.11020957174469562No Hit
GTTATTGGCAGTTGGGGATCATTACCACAAGATCACATTGTACAAAAAAT5070.10999262376882024No Hit
CTCTAGGAAGTACACCTCCTTCCCATGGCTGCTCGGTTGTGCTGCCAACT5060.10977567579294484No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAGA5030.1091248318653187No Hit
TATCAGGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTA5030.1091248318653187No Hit
TAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTTTCGAG4970.10782314401006639No Hit
CCTTTACTCAATGTGGTTATCCTGCTCTCATGCCTCTGTATGCCTGTATA4950.10738924805831561No Hit
ATACAGCCCCAAGCGACCCCGAGAGGGATCGTCCGCTGGATTCAGCGCCG4860.10543671627543715No Hit
CATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCCCTCTCACAAA4850.10521976829956177No Hit
GCCATGGGAAGGAGGTGTACTTCCTAGAGAGGACAACAGAGTTGTCTGTG4850.10521976829956177No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA4810.10435197639606021No Hit
GTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCA4790.10391808044430946No Hit
TGTCAATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACA4780.10370113246843406No Hit
CCTCCTGCTTATAGACCACCAAATACTCAAGAACTGTTTCTCTTCCAAAA4770.10348418449255868No Hit
GTTATACAGGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAA4750.1030502885408079No Hit
ATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCTTAA4730.10261639258905715No Hit
ATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTTTAATC4710.10218249663730637No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT4670.10131470473380483No Hit
CATTTGTGTATCAAATCTTGATGTAAATGAATGTCAGGAAAAGAAGGAGT4660.10109775675792945No Hit
GTTCACATTTGTGTATCAAATCTTGATGTAAATGAATGTCAGGAAAAGAA4620.10022996485442792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)