FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460940
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT28640.6213390029071029No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT28160.6109255000650844No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT23240.5041870959343949No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19860.4308586800885148No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT17750.3850826571788085No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA16420.3562285763873823No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT16100.34928624115937No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT13840.30025599861153296No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT12050.26142231092983903No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT11450.2484054323773159No Hit
ATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGAT11440.24818848440144053No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC11340.24601900464268667No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC11220.24341562893218208No Hit
GTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGG10560.22909706252440665No Hit
GTCTTAAAATAATGATTAACCACGTTATCAGGATAATAGGGTTTAATTCC9920.21521239206838202No Hit
CCATAAAGTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGT9910.21499544409250665No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG9660.20957174469562198No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG9580.20783616088861892No Hit
GGAAAATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTT9540.20696836898511736No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCAGGAAGGTCAATCACCTGGAT9450.2050158372022389No Hit
TTCCTAAGGTTACCAAATATTTTCCTATGGAAAAGGGAATTAAACCCTAT9360.20306330541936043No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA9110.1976396060224758No Hit
ATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACAAATTGTTCA8870.19243285460146656No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC8550.18549051937345426No Hit
CCTTAGGAAAAAATCTGGATGGCATAACTAATTTTAATCTTCTCAATTCA8400.18223629973532346No Hit
TAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATATTTTCCTATG8390.18201935175944808No Hit
ATATTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTGG8200.17789734021781575No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG8140.17659565236256344No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC8100.1757278604590619No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTATGGGTCACCATATT8050.174643120579685No Hit
AATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTA8050.174643120579685No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG7910.1716058489174296No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT7690.16683299344817112No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTATGGGTCACCATA7490.16249403393066342No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA7450.1616262420271619No Hit
ACTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTT7390.16032455417190958No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA7350.15945676226840805No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA7180.1557686466785265No Hit
CATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCCCTCT7140.15490085477502494No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA7050.15294832299214647No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA6870.14904325942638955No Hit
TTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTATG6760.14665683169176033No Hit
CCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCT6530.14166702824662647No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT6520.14145008027075107No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTC6510.1412331322948757No Hit
ACACAAATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAA6420.13928060051199723No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG6390.13862975658437107No Hit
GTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTTAGGAAAAAAT6320.13711112075324336No Hit
CAATAGGGCGATTTACAGGAAGTTTACGAAAACAATCTTTGATTTTTTGT6310.13689417277736798No Hit
CTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTGTTCCTACT6270.13602638087386643No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC6200.13450774504273874No Hit
GCATAACTAATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACA6190.13429079706686337No Hit
CTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTC6190.13429079706686337No Hit
GTGGTTAACTATGTCAATACTAACATGGGCCTAAAAATTAGACAATTGCT6170.1338569011151126No Hit
ATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTA6160.1336399531392372No Hit
GGCCTACAAATTGTTCACATTTGTGTATCAAATCTTGATGTAAATGAATG6030.13081962945285722No Hit
CGTTATCAGGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTG5960.1293009936217295No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA5930.12865014969410335No Hit
CTCTAGGAAGTACACCTCCTTCCCATGGCTGCTCGGTTGTGCTGCCAACT5850.12691456588710026No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA5750.12474508612834642No Hit
CTCTTTACGCGGCCTCCCCGTCTGTTCCTTCTCATCTGCCGGACCGTGTG5660.12279255434546796No Hit
ATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTTTAATC5620.12192476244196641No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC5620.12192476244196641No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA5570.12084002256258948No Hit
GTTCACATTTGTGTATCAAATCTTGATGTAAATGAATGTCAGGAAAAGAA5560.1206230745867141No Hit
ATTTACAGGAAGTTTACGAAAACAATCTTTGATTTTTTGTACAATGTGAT5490.11910443875558642No Hit
CATTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCAT5470.11867054280383564No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC5460.11845359482796027No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT5450.11823664685208486No Hit
TTTTTGTACAATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACAT5430.11780275090033411No Hit
CATTTGTGTATCAAATCTTGATGTAAATGAATGTCAGGAAAAGAAGGAGT5400.11715190697270794No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA5390.11693495899683255No Hit
GGGCTGTATCGCCCCCTTCTCCGTCTGCCGTTCCAGCCGACGACGGGTCG5290.11476547923807871No Hit
GGTTAACTCCTTCTTTTCCTGACATTCATTTACATCAAGATTTGATACAC5290.11476547923807871No Hit
CCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCA5290.11476547923807871No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5230.11346379138282639No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAGA5220.11324684340695101No Hit
GTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGAAACATAGAGGTTC5220.11324684340695101No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG5160.1119451555516987No Hit
GATTTTTTCCTAAGGTTACCAAATATTTTCCTATGGAAAAGGGAATTAAA5150.11172820757582333No Hit
GTTATTGGCAGTTGGGGATCATTACCACAAGATCACATTGTACAAAAAAT5140.11151125959994793No Hit
CTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGAC5080.11020957174469562No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTGGCAGCAGGATCTTCC5030.1091248318653187No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC4960.10760619603419101No Hit
CCTACAAATTGTTCACATTTGTGTATCAAATCTTGATGTAAATGAATGTC4940.10717230008244023No Hit
CATTCATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCC4920.10673840413068945No Hit
GGTTACCAAATATTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTG4900.1063045081789387No Hit
GTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCA4880.10587061222718792No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT4880.10587061222718792No Hit
ATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCTTAA4800.10413502842018484No Hit
TATCAGGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTA4800.10413502842018484No Hit
AGATCACATTGTACAAAAAATCAAAGATTGTTTTCGTAAACTTCCTGTAA4790.10391808044430946No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG4780.10370113246843406No Hit
TGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGATA4760.1032672365166833No Hit
ATACAGCCCCAAGCGACCCCGAGAGGGATCGTCCGCTGGATTCAGCGCCG4710.10218249663730637No Hit
CCTTTACTCAATGTGGTTATCCTGCTCTCATGCCTCTGTATGCCTGTATA4640.10066386080617867No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT4630.1004469128303033No Hit
GAATTAAACCCTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACC4630.1004469128303033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)