FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430339
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA40320.9369357645948891No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17450.4054942731195639No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT16600.38574240308222124No Hit
CACCCAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT15260.35460416090570457No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15220.3536746611392414No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG13050.3032492988086137No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13040.30301692386699786No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12080.2807089294718815No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11820.27466718098987075No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATGA11350.26374555873392835No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11140.2588656849599967No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCCTTAAATTTCAT10930.25398581118606495No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10760.25003543717859644No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAAAAC10260.2384166900978066No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT10030.23307206644064332No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAAAACCTAGCAGG9750.226565568075401No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT9060.2105316971039111No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9040.21006694722067953No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT8960.20820794768775316No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8880.20634894815482677No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG8730.20286332403058982No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8650.20100432449766348No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8220.19101220200818425No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT8060.1872942029423315No Hit
TTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7830.1819495792851682No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7810.1814848294019366No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7510.17451358115346274No Hit
CAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT7340.1705632071459942No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7260.16870420761306784No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7150.16614808325529407No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7130.16568333337206248No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6950.16150058442297816No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6930.16103583453974657No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6780.15755021041550962No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6610.1535998364080411No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6390.1484875876924936No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6310.14662858815956722No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6170.1433753389769461No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5970.13872784014463016No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATGA5910.13733359049493538No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5870.1364040907284722No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTTTGATGTGAT5850.13593934084524062No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5830.13547459096200903No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGTAGATCTCGTA5660.1315242169545405No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5630.1308270921296931No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5620.1305947171880773No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5610.1303623422464615No Hit
GTCTGGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT5560.12920046753838252No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5520.12827096777191935No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5520.12827096777191935No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTG5510.12803859283030355No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5430.12617959329737718No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC5430.12617959329737718No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5390.125250093530914No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5330.12385584388121922No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5290.12292634411475604No Hit
ATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5270.12246159423152446No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTTAAGGAATAACCCCAT5190.12060259469859809No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5150.1196730949321349No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5130.1192083450489033No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAAACAGAAA5100.11851122022405593No Hit
GTTTTATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC5090.11827884528244012No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5080.11804647034082433No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5080.11804647034082433No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG5040.11711697057436114No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAAC4980.11572272092466637No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4980.11572272092466637No Hit
GTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC4950.11502559609981898No Hit
GACATAGCCCATGAAATTTAAGGAATAACCCCATCTTTTAGTTTTGTTAG4910.1140960963333558No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGTAGATCTCGTACCGAAGGAAA4890.11363134645012421No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT4870.11316659656689261No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAAACA4870.11316659656689261No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4860.11293422162527683No Hit
CTCCGAAAGACACCAAATATTCTATGACTGTTTCTCTTCCAAAAGTGAGA4810.11177234691719784No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4810.11177234691719784No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA4800.11153997197558205No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4790.11130759703396624No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG4710.10944859750103988No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4700.10921622255942408No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4680.10875147267619249No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAAAA4660.1082867227929609No Hit
GTTATGGGTCATTGCCACAAGATCACATCAAACAGAAAATCAAAGAATGT4640.10782197290972931No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4620.10735722302649771No Hit
ATCTAGTAGATCTCGTACCGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA4620.10735722302649771No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4580.10642772326003452No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4570.10619534831841873No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4550.10573059843518715No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4550.10573059843518715No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT4510.10480109866872396No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4460.10363922396064498No Hit
TTGTATGCCTGTATTCAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTA4430.10294209913579758No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4350.10108309960287123No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC4330.10061834971963964No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4330.10061834971963964No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG4310.10015359983640805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)