FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430339
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA35270.8195864190789122No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17450.4054942731195639No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG13740.31928316978010357No Hit
CACCCAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT13330.309755797173856No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT13010.3023197990421505No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12960.3011579243340715No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12110.2814060542967288No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11700.27187868169048124No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAAAAC11640.2704844320407864No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATGA11590.2693225573327075No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11430.2656045582668547No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11120.25840093507676504No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAAAACCTAGCAGG10710.24887356247051745No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT10550.2451555634046647No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCCTTAAATTTCAT10370.2409728144555804No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT10130.23539581585680125No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9530.22145331935985352No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9410.21866482006046395No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT8750.20332807391382143No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT8500.19751870037342653No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8490.19728632543181074No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT8360.1942654511908054No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8240.19147695189141584No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8120.18868845259202627No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG8090.1879913277671789No Hit
TTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7650.1777668303360839No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7540.17521070597831012No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7520.1747459560950785No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7330.17033083220437842No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6950.16150058442297816No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6800.15801496029874124No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6650.1545293361745043No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6400.1487199626341094No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATGA6400.1487199626341094No Hit
CAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT6130.1424458392104829No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5980.13896021508624595No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5980.13896021508624595No Hit
ATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5710.13268609166261947No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5680.1319889668377721No Hit
GTCTGGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT5600.13012996730484572No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5540.12873571765515093No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGTAGATCTCGTA5460.12687671812222456No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5460.12687671812222456No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTG5420.1259472183557614No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5410.1257148434141456No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5380.12501771858929822No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTTTGATGTGAT5370.1247853436476824No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAAAA5260.12222921928990865No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5210.12106734458182968No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG5200.12083496964021387No Hit
ATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTACAAATTG5050.11734934551597694No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5020.11665222069112956No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGTAGATCTCGTACCGAAGGAAA5020.11665222069112956No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5010.11641984574951376No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4990.11595509586628218No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT4960.11525797104143477No Hit
TCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG4930.1145608462165874No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT4920.11432847127497159No Hit
ATCTAGTAGATCTCGTACCGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA4920.11432847127497159No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4910.1140960963333558No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4900.11386372139173999No Hit
GTTATGGGTCATTGCCACAAGATCACATCAAACAGAAAATCAAAGAATGT4880.1133989715085084No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4870.11316659656689261No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4860.11293422162527683No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4860.11293422162527683No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAAACA4840.11246947174204523No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4790.11130759703396624No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4790.11130759703396624No Hit
GTTTTATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC4770.11084284715073464No Hit
CATTGGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA4770.11084284715073464No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4760.11061047220911886No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4610.10712484808488192No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4610.10712484808488192No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4580.10642772326003452No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4550.10573059843518715No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAAACAGAAA4540.10549822349357135No Hit
CTCCGAAAGACACCAAATATTCTATGACTGTTTCTCTTCCAAAAGTGAGA4530.10526584855195555No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4530.10526584855195555No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAAC4530.10526584855195555No Hit
TTGTATGCCTGTATTCAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTA4510.10480109866872396No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTTAC4470.10387159890226078No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4470.10387159890226078No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4460.10363922396064498No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4430.10294209913579758No Hit
GTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC4390.10201259936933442No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT4360.10131547454448701No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4340.10085072466125543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)