FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520837
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32560.6251475989609033No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT16060.30834982921720233No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14550.2793580333194454No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAA12950.24863824958672293No Hit
TCCACAGAGTGTGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA12880.2472942590484163No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12760.24499027526846212No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12180.23385435366535018No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT11860.2277103969188057No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11210.21523048477738718No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9820.1885426726595845No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8970.17222278755157563No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8840.16972680512329194No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8490.1630068524317589No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAGGTAAGA8270.15878288216850953No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8220.15782288892686194No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCA8050.1545589119052602No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7930.152254928125306No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7690.1476469605653976No Hit
GTGTGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7640.14668696732375006No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7600.14591897273043197No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7490.1438069875988073No Hit
GTGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7450.14303899300548925No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7430.14265499570883022No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7350.14111900652219408No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7160.13747103220393328No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7010.13459105247899053No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6880.13209507005070684No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6780.1301750835674117No Hit
CATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAAGGAGCAGCA6670.12806309843578703No Hit
CCTTTTACACAATGTGGTTATCCCGCCTTGATGCCTTTATATGCATGTAT6610.12691110654580992No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6530.1253751173591738No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6410.12307113357921959No Hit
ACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATTTTCC6350.1219191416892425No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6340.121727143040913No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6300.12095914844759492No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA6250.11999915520594735No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6200.11903916196429978No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6150.11807916872265219No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6080.11673517818434559No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5930.11385519845940285No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5900.1132792025144143No Hit
CCTATTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA5860.11251120792109624No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5850.11231920927276672No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5820.11174321332777816No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGTCTAGT5820.11174321332777816No Hit
ATATAAAAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT5750.11039922278947158No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5690.10924723089949445No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5610.10771124171285835No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5590.10732724441619931No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5590.10732724441619931No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5560.10675124847121076No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5520.1059832538778927No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5510.10579125522956317No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5500.10559925658123366No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5460.1048312619879156No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5310.10195128226297287No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5220.10022329442800723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)