FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520837
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32430.6226516165326196No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14630.2808940225060816No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT13870.26630212523303837No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAA13470.25862217929985776No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12760.24499027526846212No Hit
TCCACAGAGTGTGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA12040.23116637258873696No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11520.22118244287560213No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11170.2144624901840691No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10510.20179057939432107No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10440.20044658885601446No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9430.18105472537473338No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9400.18047872942974483No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAGGTAAGA9180.17625475916649547No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9020.17318278079322322No Hit
GTGTGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8660.16627082945336064No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8610.16531083621171308No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8430.1618548605417818No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8250.15839888487185053No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCA7960.15283092407029455No Hit
GTGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7750.14879895245537472No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7470.14342299030214828No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7380.14169500246718264No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7140.13708703490727425No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7100.1363190403139562No Hit
CCTTTTACACAATGTGGTTATCCCGCCTTGATGCCTTTATATGCATGTAT7080.13593504301729714No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6860.1317110727540478No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6850.1315190741057183No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6810.13075107951240023No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6780.1301750835674117No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6700.12863909438077556No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6680.12825509708411653No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6630.12729510384246895No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6570.12614311195249187No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGTCTAGT6540.1255671160075033No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6530.1253751173591738No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6450.12383912817253767No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6310.12115114709592445No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6200.11903916196429978No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG6130.11769517142599317No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5910.11347120116274381No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5840.1121272106244372No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5840.1121272106244372No Hit
ACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATTTTCC5680.10905523225116497No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5630.10809523900951737No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5630.10809523900951737No Hit
CATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAAGGAGCAGCA5570.10694324711954027No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5540.10636725117455173No Hit
ATATAAAAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT5470.10502326063624512No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5280.10137528631798433No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5240.10060729172466626No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5210.10003129577967772No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5210.10003129577967772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)