FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448992
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23120.5149312237189081No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC19910.44343774499322924No Hit
GATCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAA18340.4084705295417291No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16030.3570219513933433No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG14530.32361378376452143No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13890.30935963224289076No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11540.2570201696244031No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10950.2438796236903998No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10910.24298873922029793No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10310.22962547216876916No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9710.2162622051172404No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT9580.21336683058940917No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAACCTTAT9270.20646247594611927No Hit
AAGCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTT9160.20401254365333904No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8540.19020383436675933No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8470.18864478654408096No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8450.18819934430903001No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8060.1795132207255363No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7960.1772860095502815No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT7360.16392274249875277No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7340.1634773002637018No Hit
CTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG7200.1603592046183451No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7100.15813199344309029No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7050.1570183878554629No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6970.15523661891525906No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTACGCGGGACGTCC6920.15412301332763167No Hit
CTTATATAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA6840.15234124438742783No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6780.15100491768227498No Hit
AAATCGACCTATTGATTGGAAAGTCTGTCAAAGAATTGTGGGTCTTTTGG6600.14699593756681634No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6570.1463277742142399No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6560.14610505309671443No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6480.14432328415651058No Hit
AAATAGACCTATTGATTGGAAAGTCTGTCAAAGAATTGTGGGTCTTTTGG6450.14365512080393414No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6440.14343239968640867No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6270.13964614068847553No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC6230.1387552562183736No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6220.13853253510084812No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6220.13853253510084812No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6040.1345235549853895No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6020.13407811275033854No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5940.1322963438101347No Hit
ATGTTAATATGGGCCTAAAAATCAGACAGCTATTGTGGTTCCACATTTCC5900.13140545934003278No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG5890.1311827382225073No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5880.13096001710498184No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5870.13073729598745634No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5860.13051457486993087No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5820.12962369039982896No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5660.1260601525194213No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5590.12450110469674291No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5480.12205117240396265No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5480.12205117240396265No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5470.12182845128643717No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5410.1204921245812843No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5370.11960124011118238No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5270.11737402893592759No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT5250.11692858670087664No Hit
ATACTGCGGAGCTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCG5210.11603770223077473No Hit
GCTATTGTGGTTCCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTC5210.11603770223077473No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5200.11581498111324924No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5050.11247416435036703No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGTTGTCAGTC5020.1118060009977906No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5000.11136055876273965No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5000.11136055876273965No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAA4990.11113783764521418No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4990.11113783764521418No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4980.11091511652768868No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4960.11046967429263774No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4930.10980151094006128No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA4920.10957878982253583No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCGCGCAGGCGCCGATGGCC4910.10935606870501034No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4820.10735157864728102No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4820.10735157864728102No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4780.1064606941771791No Hit
CTTATGTAAGAGCTTGGGCAAGACCTTGGGCAAGACCTGGTGGGCGTTCA4740.10556980970707719No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4740.10556980970707719No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4730.1053470885895517No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4690.10445620411944978No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4680.1042334830019243No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4660.10378804076687335No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4650.10356531964934787No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4610.10267443517924596No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4610.10267443517924596No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAAC4600.10245171406172048No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4580.10200627182666952No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4570.10178355070914404No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4560.10156082959161855No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4520.10066994512151664No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4490.1000017817689402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)