FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731060
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG40270.5508439799742839No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38960.5329247941345444No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT29520.40379722594588685No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG27770.3798593822668454No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT27740.37944901923234753No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22680.3102344540803765No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA18540.2536043553196728No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG17490.23924164911224796No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16740.22898257324980167No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT16600.22706754575547833No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15940.21803955899652558No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA15750.21544059311137254No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA15610.21352556561704922No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA15010.20531830492709216No Hit
GTCCCATGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC14710.20121467458211365No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14370.19656389352447132No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG14350.1962903181681394No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT13550.18534730391486337No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG12710.1738571389489235No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA12320.1685224195004514No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12050.16482915218997074No Hit
GCATGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG11850.16209339862665173No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC11780.16113588487949004No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11120.1521078981205373No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG10990.15032965830437994No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG10940.1496457199135502No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGT10790.14759390474106093No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC10440.14280633600525264No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10380.14198560993625695No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10290.14075452083276338No Hit
GATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC10270.1404809454764315No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10150.13883949333844006No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCC9980.1365141028096189No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9960.13624052745328702No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9930.13583016441878915No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG9930.13583016441878915No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA9870.13500943834979345No Hit
CTCCAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9490.1298115065794873No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9490.1298115065794873No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA9480.12967471890132137No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9340.12775969140699805No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9260.12666538998167046No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9180.12557108855634286No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9040.12365606106201953No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8970.12269854731485788No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA8870.12133067053319838No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8780.12009958142970481No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA8690.11886849232621126No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8630.11804776625721554No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8620.1179109785790496No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGGTT8590.11750061554455175No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT8570.11722704018821985No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG8330.11394413591223702No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT8280.11326019752140727No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTCCGAAAAC8270.1131234098432413No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8200.11216589609607966No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT8190.11202910841791372No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8190.11202910841791372No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG8110.11093480699258611No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG8090.11066123163625421No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA8080.11052444395808826No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC8070.1103876562799223No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT8060.11025086860175635No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTCGACATTGCTGAGAGTCCAA8050.1101140809235904No Hit
ATGTTAATGCGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7880.10778869039476924No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAATTGCCTGTAA7860.10751511503843734No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7850.10737832736027139No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7690.10518972450961617No Hit
GGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC7590.10382184772795666No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTTCACCAAGCTCTGATAG7590.10382184772795666No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATGAGGGTTCAAATGTATA7490.10245397094629716No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC7460.1020436079117993No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7380.10094930648647171No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7350.10053894345197385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)