FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465161
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA17080.36718469519155733No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT16650.3579405840128472No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC14370.30892529683270953No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14210.3054856275569104No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13250.2848476119021156No Hit
ATCCAGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAC12960.2786132113397297No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAA12820.27560350072340545No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG12690.27280876943681864No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATCAAGGCGGGATAACCACAT11270.24228170461410137No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11180.24034689064646436No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11000.2364772627111903No Hit
ATATATTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA10810.23239265544617885No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10500.22572829622431803No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10310.22164368895930656No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10270.22078377164035679No Hit
GTTCTGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA10140.21798904035376995No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10120.2175590816942951No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA9990.21476435040770828No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9930.2134744744292836No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT9740.2093898671642722No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT9300.19993077665582454No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCACAG9200.1977809833584501No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9060.19477127274212586No Hit
GTGTGTAGTTTCTCTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTA9020.19391135542317606No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8950.19240650011501395No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT8950.19240650011501395No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAA8780.18875185150947738No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8780.18875185150947738No Hit
GGAATATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT8610.18509720290394077No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8560.18402230625525356No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8440.1814425542984042No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8440.1814425542984042No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGATACTCCCTTAACTTCAT8260.17757292636313018No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT8170.17563811239549318No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAC8060.17327333976838127No Hit
CCTTTTACACAATGTGGTTATCCCGCCTTGATGCCTTTATATGCATGTAT7760.1668239598762579No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7630.16402922858967112No Hit
GTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTTTTGTC7590.16316931127072132No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7570.16273935261124645No Hit
CCTGCAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG7330.15757984869754774No Hit
ATATTAACGTTGACATAGCTGACTACTGCTTCCCTGGATGCTGGGTCTTC7300.1569349107083354No Hit
ATATAAAAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT7200.15478511741096093No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7070.15199038612437415No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6990.15027055148647456No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6870.14769079952962522No Hit
CAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTG6840.14704586154041288No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCTGG6810.14640092355120055No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG6750.14511104757277588No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6600.1418863576267142No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6550.14081146097802696No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACACAGCGCT6470.1390916263401274No Hit
CATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAAGGAGCAGCA6420.1380167296914402No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6400.13758677103196526No Hit
GTGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAAGTTAAGGGAG6250.13436208108590358No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6210.1335021637669538No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6180.13285722577774148No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA6170.13264224644800401No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6170.13264224644800401No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6080.130707432480367No Hit
GTTCATCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAG6040.12984751516141724No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5960.12812768052351767No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5920.1272677632045679No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5900.12683780454509297No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5850.12576290789640576No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5740.12339813526929386No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA5650.12146332130165685No Hit
CTTTTGGAGTGTGGATTCGCACTCCTATCGCTTACAGACCACCAAATGCC5620.12081838331244452No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5610.12060340398270707No Hit
GTTAAGGGAGTATCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATAC5580.11995846599349473No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5510.1184536106853326No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5420.11651879671769559No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT5410.11630381738795814No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5410.11630381738795814No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5390.11587385872848326No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5390.11587385872848326No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAAT5350.11501394140953347No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5290.1137240654311088No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5260.11307912744189647No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5260.11307912744189647No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5250.11286414811215902No Hit
CTCCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCAC5230.11243418945268413No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5230.11243418945268413No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5230.11243418945268413No Hit
AGGCCAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACCCCACCA5180.1113592928039969No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5150.11071435481478456No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5110.10985443749583478No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5070.10899452017688498No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5040.10834958218767264No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5010.1077046441984603No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTC5000.10748966486872288No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT5000.10748966486872288No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4960.1066297475497731No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4960.1066297475497731No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4950.10641476822003564No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTG4880.10490991291187352No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4870.1046949335821361No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT4850.10426497492266118No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4830.1038350162631863No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGATACTCCCTTAAC4810.1034050576037114No Hit
GTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCTGGAT4800.10319007827397396No Hit
GGCCTATATATTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT4780.10276011961449906No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4770.10254514028476162No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAG4760.10233016095502417No Hit
GTTTAGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA4760.10233016095502417No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGTGTGTAGTTTCTCTTTTATA4730.10168522296581184No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4730.10168522296581184No Hit
ATTTACACACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4730.10168522296581184No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4710.10125526430633694No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC4690.10082530564686205No Hit
GTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGACAAATATT4690.10082530564686205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)