FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465161
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA16970.36481992256444545No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15770.33902240299595193No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG14770.31752447002220735No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14560.313009904097721No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAA13140.2824828392750037No Hit
ATCCAGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAC12700.2730237487665561No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12460.26786424485285737No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11830.2543205470793983No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATCAAGGCGGGATAACCACAT11770.2530306711009736No Hit
ATATATTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA11440.24593635321963794No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10900.23432746941381585No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA10630.22852302751090484No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10510.22594327555405547No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10300.22142870962956912No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10140.21798904035376995No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10030.2156242677266581No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCACAG9950.21390443308875853No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9930.2134744744292836No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT9480.20380040459109855No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAA9460.20337044593162368No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT9340.20079069397477434No Hit
GTGTGTAGTTTCTCTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTA9250.1988558800071373No Hit
GTTCTGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA9190.19756600402871263No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8890.19111662413658928No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8760.18832189285000248No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT8690.18681703754184037No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8670.18638707888236544No Hit
GGAATATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT8600.18488222357420336No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8360.17972271966050465No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8340.17929276100102975No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT8190.17606807105496808No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8060.17327333976838127No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGATACTCCCTTAACTTCAT8040.1728433811089064No Hit
CCTTTTACACAATGTGGTTATCCCGCCTTGATGCCTTTATATGCATGTAT7990.17176848446021914No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7720.16596404255730812No Hit
ATATTAACGTTGACATAGCTGACTACTGCTTCCCTGGATGCTGGGTCTTC7390.1588697246759724No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAC7360.15822478668676007No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7360.15822478668676007No Hit
CCTGCAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG7240.15564503472991073No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7230.15543005540017327No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCTGG7160.15392520009201116No Hit
GTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTTTTGTC7100.1526353241135865No Hit
CATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAAGGAGCAGCA6880.14790577885936268No Hit
CAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTG6870.14769079952962522No Hit
ATATAAAAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT6830.14683088221067545No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6790.14597096489172565No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6770.14554100623225077No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACACAGCGCT6590.14167137829697674No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6590.14167137829697674No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6580.1414563989672393No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6410.13780175036170272No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA6330.13608191572380315No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6230.13393212242642868No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAAT6220.13371714309669125No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6220.13371714309669125No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6190.1330722051074789No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6180.13285722577774148No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6140.13199730845879168No Hit
CTTTTGGAGTGTGGATTCGCACTCCTATCGCTTACAGACCACCAAATGCC6120.1315673497993168No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6100.1311373911398419No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6050.13006249449115467No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6030.12963253583167977No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG6030.12963253583167977No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5960.12812768052351767No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5930.1274827425343053No Hit
GTTCATCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAG5820.12511796990719343No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5800.12468801124771853No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA5750.1236131145990313No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5730.12318315593955641No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC5710.12275319728008152No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5710.12275319728008152No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5690.12232323862060664No Hit
GTTAAGGGAGTATCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATAC5660.1216783006313943No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5650.12146332130165685No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT5550.1193135280042824No Hit
CTCCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCAC5540.11909854867454493No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5530.11888356934480751No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5500.11823863135559515No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5370.11544390006900836No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5360.11522892073927092No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5340.11479896207979602No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5320.11436900342032114No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5260.11307912744189647No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT5230.11243418945268413No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5220.11221921012294668No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5210.11200423079320923No Hit
GTGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAAGTTAAGGGAG5160.11092933414452201No Hit
ATTTACACACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5130.11028439615530967No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5130.11028439615530967No Hit
GGCCTATATATTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT5120.11006941682557222No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5110.10985443749583478No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTG5060.10877954084714755No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5030.10813460285793522No Hit
GTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCTGGAT4990.10727468553898543No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4940.10619978889029819No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4930.10598480956056076No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4910.10555485090108585No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4910.10555485090108585No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4900.10533987157134843No Hit
CTTCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4890.10512489224161096No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4840.10404999559292373No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4810.1034050576037114No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4720.10147024363607439No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG4720.10147024363607439No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTC4710.10125526430633694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)