FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419281
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA33710.8039954111920168No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG29910.7133640684886746No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT27950.6666173759364246No Hit
CTATCAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG22390.5340094113494291No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG19810.4724754997245284No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18950.4519641958495615No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG17910.4271598283728573No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG15740.3754045616185804No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15080.35966332841221044No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14680.3501231870750165No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC14530.3465456340735688No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14500.34583012347327924No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14360.3424910740052614No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14300.3410600528046823No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14250.33986753513753304No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAA12990.30981608992537224No Hit
GTACAGAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT12310.2935978496521426No Hit
GTTTCTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA12210.2912128143178441No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT12170.29025880018412475No Hit
TTATTGATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA10670.25448327016964756No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10610.25305224896906847No Hit
ATCCCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10600.25281374543563867No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10580.25233673836877896No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10550.2516212277684894No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10060.23993455463042684No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCATGT9980.23802652636298807No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9910.23635700162897913No Hit
AAATCAGACAACTATTGTGGTTTCACATTTCCTGCCTTACTTTTGGAAGA9890.23587999456211947No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9480.22610134969149567No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT9180.21894624368860022No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8990.21441467655343313No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8990.21441467655343313No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAATGACACCAAATACTCAAGAAC8440.2012969822147915No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG8400.20034296808107213No Hit
GTCTTATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCC8280.19748092567991393No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7800.18603275607528127No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7750.18484023840813202No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7730.18436323134127233No Hit
CTCCAAATGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG7700.1836477207409828No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7580.18078567833982462No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7530.17959316067267536No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7510.17911615360581568No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATC7390.1762541112046575No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA7360.17553860060436796No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7320.17458458647064856No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7230.17243805466977993No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG7180.1712455370026307No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7090.16909900520176205No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7070.16862199813490236No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7040.16790648753461285No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6790.16194389919886662No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6770.16146689213200693No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA6650.15860484973084876No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG6600.15741233206369953No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6560.15645831792998013No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6280.1497802189939444No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6250.14906470839365485No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6200.14787219072650562No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA6160.14691817659278622No Hit
GTTTCATTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6140.1464411695259265No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG6040.14405613419162805No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5770.13761653878902216No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5770.13761653878902216No Hit
GCCTACGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5710.13618551758844305No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5660.13499299992129382No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5650.134754496387864No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5650.134754496387864No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAA5640.13451599285443414No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5630.1342774893210043No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5610.1338004822541446No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5520.13165395045327596No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT5480.13069993631955656No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5430.12950741865240734No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5400.1287919080521178No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5390.12855340451868794No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5340.1273608868515387No Hit
GCTTTATTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCT5260.1254528585840999No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5240.12497585151724022No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5150.1228293197163716No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT5110.12187530558265222No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC5100.12163680204922236No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4960.1182977525812045No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4870.11615122078033586No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4850.11567421371347615No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4840.11543571018004632No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4840.11543571018004632No Hit
ATACAGCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTG4790.11424319251289708No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4760.11352768191260754No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4750.1132891783791777No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4670.11138115011173891No Hit
AAGCGGTAGGAGTGCGAATCCACACTCCAAATGACACCAAATACTCAAGA4640.11066563951144935No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4610.10995012891115982No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4580.10923461831087028No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4580.10923461831087028No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCAACCTGGGTGGGAAGTAATTTGG4570.10899611477744042No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4550.10851910771058074No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4490.10708808651000165No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4480.1068495829765718No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4440.10589556884285241No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATA4420.10541856177599272No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4410.10518005824256287No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4410.10518005824256287No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4390.10470305117570317No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4390.10470305117570317No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4370.10422604410884348No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4340.10351053350855394No Hit
CTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCT4320.10303352644169424No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4320.10303352644169424No Hit
GCGTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTA4250.10136400170768531No Hit
TATTGATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAA4250.10136400170768531No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4250.10136400170768531No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4240.10112549817425545No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4230.10088699464082561No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4220.10064849110739575No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCAGATGGCC4210.1004099875739659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)