FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519339
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG22170.426888795180027No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21510.41418033307723856No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17330.3336934064262457No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11350.21854703767673908No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11130.2143108836424763No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11040.2125779115375506No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10910.2100747296082135No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9910.19081948399792814No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9350.18003654645616832No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7960.15327175505787163No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7690.14807283874309457No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7620.14672497155037462No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7520.14479944698934608No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7450.1434515797966261No Hit
CTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTCCTAAC7360.14171860769170042No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7020.13517182418420337No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6840.131705879974352No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCTTCATTTTGTGGGTCACCATA6510.12535164892295783No Hit
TTCTTCTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTC6420.12361867681803214No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6320.12169315225700361No Hit
TTCCTAACCTTACCAAATATTTGCCATTGGATAAAGGCATTAAACCTTAT6270.12073038997648936No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5830.11225808190796378No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5790.11148787208355236No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5680.10936979506642097No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA5570.10725171804928958No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5510.10609640331267246No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5410.10417087875164391No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5280.10166769682230681No Hit
GGATAATAAGGTTTAATGCCTTTATCCAATGGCAAATATTTGGTAAGGTT5260.10128259191010112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)