FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403976
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT20660.511416519793255No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG16510.40868764481058284No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16320.40398439511258094No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13550.3354159653048696No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12820.3173455848862308No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11420.2826900607956908No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11240.27823435055547857No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT10520.2604115095946294No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA10450.2586787333901024No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10350.25620333881220664No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9710.2403608135136741No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9620.23813295839356796No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9450.2339247876111452No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8880.21981503851713963No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8880.21981503851713963No Hit
GTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA8180.2024872764718696No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7910.1958037111115512No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7610.18837752737786403No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7520.1861496722577579No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7470.18491197496881No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7430.18392181713765174No Hit
ATGTCAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7340.1816939620175456No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7240.1792185674396499No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7080.17525793611501672No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCC6980.17278254153712103No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6960.17228746262154188No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6950.1720399231637523No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6870.17005960750143573No Hit
TATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAA6870.17005960750143573No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA6840.169316989128067No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6730.16659405509238173No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA6580.16288096322553813No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6440.15941541081648414No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6240.1544646216606927No Hit
CATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG6020.14901875358932212No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6000.148523674673743No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5960.1475335168425847No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC5860.145058122264689No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5750.1423351882290037No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5630.13936471473552883No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTCATTACAATA5560.13763193853100184No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5480.13565162286868526No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5430.13441392557973741No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5390.13342376774857911No Hit
GTTCCGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5350.13243360991742084No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACAT5320.13169099154405212No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5310.13144345208626257No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5270.13045329425510427No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTT5250.12995821533952512No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5220.1292155969661564No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5140.12723528130383985No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5090.12599758401489197No Hit
ATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA5010.12401726835257539No Hit
CCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC4870.1205517159435214No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTCATT4830.11956155811236312No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4810.11906647919678398No Hit
AAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA4760.1178287819078361No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4730.11708616353446741No Hit
GTCCGGCACATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4710.11659108461888824No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC4700.11634354516109868No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4690.11609600570330911No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTCATTACAAT4670.11560092678772997No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4660.1153533873299404No Hit
GCTTTAGGGCATGGACATCGACCCCTATAAAGAATTTGGAGCTTCTGTGG4620.1143632294987821No Hit
TCTGTGGAGTTGCTCTCTTTTTTGCCTTCTGACTTCTTTCCCGCTATTCG4620.1143632294987821No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4570.11312553220983425No Hit
GGATAAAGGCATTAAACCTTATTATCCGGAACATGCAGTTAATCATTACT4570.11312553220983425No Hit
GTTTCCGAAAACATTGCTTAATTTTCATTACAATATGTTCCTGTGGTAAA4530.11213537437867596No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4520.11188783492088639No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4520.11188783492088639No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTCATTACAATATGTT4500.11139275600530725No Hit
GCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4450.1101550587163594No Hit
ATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGAATCTG4420.10941244034299068No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGT4380.10842228251183239No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4370.1081747430540428No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4340.10743212468067409No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4330.10718458522288453No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4270.10569934847614709No Hit
CTGTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC4250.10520426956056796No Hit
GTCTTCCAAATTACCTCCCACCCAGGTGGCCAGATTCATCAACTCACCCC4180.10347149335604094No Hit
GGAACATATTGTAATGAAAATTAAGCAATGTTTTCGGAAACTGCCTGTAA4160.10297641444046181No Hit
TCCCATGGCTGCTCGGATGTACTGCCAACTGGATCCTGCGCGGGACGTCC4090.1012436382359348No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAATAAGGTTTAATGCC4040.10000594094698695No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4040.10000594094698695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)