FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403976
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG18970.4695823514268174No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT16950.419579380953324No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16200.4010139216191061No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12590.31165217735707074No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12520.30991940115254374No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT10740.265857377666No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10530.260659049052419No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10370.25669841772778584No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10150.25125254965641525No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA10020.24803453670515077No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9530.2359051032734618No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9380.23219201140661822No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9330.23095431411767034No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8920.2208051963482979No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8480.20991346020555676No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8420.20842822345881934No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8100.20050696080955305No Hit
GTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA7770.19233815870249718No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7450.1844168960532309No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7250.17946610689743944No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7200.1782284096084916No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7150.17699071231954375No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7110.17600055448838545No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7000.17327762045270015No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCC6770.16758421292354No Hit
ATGTCAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6720.16634651563459216No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6550.16213834485216944No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA6370.15768263461195714No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT6310.15619739786521972No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6270.15520724003406142No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6120.15149414816721787No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTCATTACAATA5870.14530566172247858No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5850.1448105828068994No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA5760.14258272768679328No Hit
GTTCCGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5750.1423351882290037No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC5630.13936471473552883No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5550.13738439907321226No Hit
ATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA5510.136394241242054No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5440.134661465037527No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5400.13367130720636872No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5350.13243360991742084No Hit
TATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAA5190.1284729785927877No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5190.1284729785927877No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5180.12822543913499812No Hit
CATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG5110.12649266293047112No Hit
GTCTTCCAAATTACCTCCCACCCAGGTGGCCAGATTCATCAACTCACCCC5070.12550250509931282No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4970.12302711052141711No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTCATTACAAT4920.12178941323246927No Hit
GTTTCCGAAAACATTGCTTAATTTTCATTACAATATGTTCCTGTGGTAAA4810.11906647919678398No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACAT4750.11758124245004654No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4670.11560092678772997No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4620.1143632294987821No Hit
CCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC4610.11411569004099253No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTT4560.11287799275204469No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4550.11263045329425511No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4540.11238291383646552No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4500.11139275600530725No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4500.11139275600530725No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTCATTACAATATGTT4470.11065013763193854No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4420.10941244034299068No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC4410.1091649008852011No Hit
GTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA4340.10743212468067409No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG4330.10718458522288453No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4300.10644196684951582No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4290.10619442739172623No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4270.10569934847614709No Hit
GCTTTAGGGCATGGACATCGACCCCTATAAAGAATTTGGAGCTTCTGTGG4180.10347149335604094No Hit
GTCCGGCACATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4170.1032239538982514No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4160.10297641444046181No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4150.10272887498267223No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGT4150.10272887498267223No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAATAAGGTTTAATGCC4080.10099609877814524No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4070.10074855932035566No Hit
AAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA4040.10000594094698695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)