FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599519
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG39240.6545247106430322No Hit
GATTTAATAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA29820.4973987479963104No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT28860.48138591103868267No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG25390.42350617745225755No Hit
GTGTAAAAGGAGCAGCAAAGCCTAAAAGACCCACAATTCTTTGACATACT25370.423172576682307No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT23210.38714369352764466No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC22060.36796164925548647No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT20260.33793757995993456No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGA19610.3270955549365408No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCA18770.31308432259861657No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC16570.2763882379040531No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA16440.27421983289937435No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG16310.27205142789469555No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT15990.26671381557548635No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15980.26654701519051105No Hit
GCCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT15490.2583737963267219No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA14410.24035935474939077No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG14200.23685654666490968No Hit
CCTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT14080.23485494204520627No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC13850.2310185331907746No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13820.23051813203584876No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13200.22017650816738085No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12540.20916768275901182No Hit
ATTGATAGGTGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAAAG12400.20683247736935778No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA12000.20016046197034623No Hit
CAATACGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT11830.1973248554257663No Hit
CTTTTATCTCTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGG11710.1953232508060629No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA11650.19432244849621114No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC10780.17981081500336102No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10740.1791436134634599No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10670.17797601076863287No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA10640.17747560961370698No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA10130.16896878997996728No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9830.1639647784307086No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9760.1627971757358816No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9660.1611291718861287No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9600.160128369576277No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9550.15929436765140054No Hit
TTATTAATAGGTGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAA9480.15812676495657352No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9360.15612516033687004No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA9200.15345635417726544No Hit
CTACTAACTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG9170.15295595302233958No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATA9140.15245555186741372No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT9110.15195515071248783No Hit
ATTAATAGGTGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAAAG9070.15128794917258667No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8970.1496199453228338No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8800.14678433877825392No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8750.14595033685337744No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC8620.14378193184869872No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8580.14311473030879757No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8580.14311473030879757No Hit
ATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA8550.1426143291538717No Hit
GAGTTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA8510.14194712761397055No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAAT8460.1411131256890941No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT8390.13994552299426707No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8330.13894472068441535No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCCATGTCAACGACCGACCT8230.13727671683466247No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTAT7990.13327350759525552No Hit
GGATAGCCACATTGTGTAAAAGGAGCAGCAAAGCCTAAAAGACCCACAAT7850.1309383022056015No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG7820.13043790105067563No Hit
TAGCCACATTGTGTAAAAGGAGCAGCAAAGCCTAAAAGACCCACAATTCT7820.13043790105067563No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7800.13010430028072506No Hit
CTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7700.12843629643097215No Hit
ATGAAGCACTGCGTGTAGTTTCTCTCTTATATAGGATGCCAGCCTTCCAC7660.12776909489107102No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7640.12743549412112043No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC7630.12726869373614513No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA7490.1249334883464911No Hit
CCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGACA7450.12426628680658994No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC7300.12176428103196063No Hit
GAATATGGTGACCCACAAAATGAAGCACTGCGTGTAGTTTCTCTCTTATA7180.11976267641225717No Hit
CTGTAAGAGGGCCCACGTATTGTTGACACCTATCAATAATGTCCTCCTGT7090.11826147294747957No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCAAAG7050.11759427140757842No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6930.11559266678787496No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6920.11542586640289965No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG6890.1149254652479738No Hit
CTTTACCACAGGAACATATTGTACAAAAACTCAAGCAATGTTTTCGAAAA6670.11125585677851743No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATATGTT6660.11108905639354216No Hit
ATATTGTACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA6610.11025505446866572No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC6610.11025505446866572No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTC6540.1090874517738387No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6460.10775304869403639No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6320.10541784330438235No Hit
AAATGAAGCACTGCGTGTAGTTTCTCTCTTATATAGGATGCCAGCCTTCC6300.10508424253443177No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT6300.10508424253443177No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT6270.10458384137950591No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA6250.10425024060955533No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6250.10425024060955533No Hit
ATTGATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6190.1032494382997036No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6110.10191503521990129No Hit
ATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATATT6070.10124783368000013No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6030.10058063214009898No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6000.10008023098517312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)