FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671145
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG34550.5147918855090926No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT27660.412131506604385No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA27090.4036385579867242No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT24340.3626638058839744No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG22140.3298840042017746No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA19630.2924852304643557No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18800.280118305284253No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT18070.2692413710897049No Hit
CATCTATTAATAATGTCCTCCCGTAAATGAATGTTAGGAAAGGAGGGAGT17710.2638774035417086No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT16960.2527024711500495No Hit
TGATCGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA15820.2357165739147278No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15300.22796862078984423No Hit
CCCTTAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACTTTACCACAG14290.21291971183574337No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAACCCATGAA13500.20114878304986253No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC13480.20085078485275165No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12210.181927899336209No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA12140.18088490564632084No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11460.17075296694454997No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11330.1688159786633291No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA11070.1649420021008873No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG10970.16345201111533275No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG10820.16121702463700094No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG10690.15928003635578006No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC10420.1552570606947828No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC10090.15034009044245283No Hit
GGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTT10000.14899909855545374No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAAGGTT9940.14810510396412102No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9930.14795610486556557No Hit
CTATTAATAATGTCCTCCCGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9770.1455721192886783No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9520.14184714182479194No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9350.13931415714934925No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9210.13722816976957292No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9200.13707917067101744No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA9200.13707917067101744No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8880.1323111995172429No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATAACCCATGAAGTTAAGGGAG8810.13126820582735474No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG8540.1272452301663575No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACGGG8460.12605323737791388No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8430.12560624008224752No Hit
ATCCAAGGGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAA8400.12515924278658114No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAACCC8390.12501024368802569No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATC8370.12471224549091477No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8370.12471224549091477No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8340.12426524819524841No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCCGT8230.12262625811113843No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8130.1211362671255839No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG8070.12024227253425115No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8010.11934827794291844No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7930.11815628515447481No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7890.11756028876025301No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7760.11562330047903209No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA7630.1136863121978112No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7490.11160032481803484No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT7450.11100432842381304No Hit
GTTTTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC7370.1098123356353694No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7350.1095143374382585No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA7350.1095143374382585No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7310.10891834104403669No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT7180.1069813527628158No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7170.10683235366426033No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7170.10683235366426033No Hit
ATCCTAACCTTACCAAATATTTACCTTTGGATAAAGGCATTAAACCTTAT7040.10489536538303944No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7030.10474636628448397No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7000.10429936898881761No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA6960.1037033725945958No Hit
GCTTTATTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCT6940.1034053743974849No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6890.10266037890470762No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6890.10266037890470762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)