FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431345
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20040.46459330698165036No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAAAG15660.3630504584497328No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12720.2948915601200895No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12510.2900230673822578No Hit
GTGTAAATGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12030.2788950839814997No Hit
CTCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACTCTACCACAG11410.26452143875552053No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10650.2469021317043202No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10500.24342463689158328No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10400.24110630701642535No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10290.23855614415375162No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA9900.22951465764063567No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9370.21722750930229864No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAT9340.21653201033975125No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9330.21630017735223545No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCTTAT9040.20957702071427745No Hit
CCCTCATAAAACAAAACGTTGGGGCTACTCTCTTAACTTCATGGGATATG8830.2047085279764458No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTAAGGTT8800.2040130290138984No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8680.20123103316370886No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8620.1998400352386141No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT8490.19682620640090875No Hit
ATATGTTCCTGTGGTAGAGTACCCCAACTTCCAATGACATATCCCATGAA7540.17480207258690839No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC7430.17225190972423465No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7300.16923808088652934No Hit
GTACAATATGTTCCTGTGGTAGAGTACCCCAACTTCCAATGACATATCCC7230.1676152499739188No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA7040.16321042321111873No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAATGGGGCAGCA6890.1597329283983818No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAA6700.15532810163558172No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6590.152777938772908No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA6580.1525461057853922No Hit
GTGGTAGAGTACCCCAACTTCCAATGACATATCCCATGAAGTTAAGAGAG6540.151618773835329No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6420.1488367779851395No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6320.14651844810998155No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGT6260.1451274501848868No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6240.1446637842098552No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC6120.1418817883596657No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCA5990.13886795952196038No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5920.13724512860934984No Hit
CTCTACCACAGGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAA5890.13654962964680245No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA5820.1349267987341919No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATATGTT5810.1346949657466761No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5760.13353580080909713No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5750.13330396782158135No Hit
CATTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5700.13214480288400238No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5640.13075380495890762No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT5630.13052197197139181No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5620.130290138983876No Hit
CATTTGAACCCTAATAAAACAAAACGTTGGGGCTACTCTCTTAACTTCAT5580.12936280703381284No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5530.12820364209623386No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5440.1261171452085917No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5350.1240306483209496No Hit
GTTTTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC5240.12148048545827586No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5230.12124865247076008No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5220.12101681948324426No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5130.11893032259560213No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTA5130.11893032259560213No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4940.11452549583280205No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4930.11429366284528626No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4880.1131344979077073No Hit
AGTTAAGAGAGTAGCCCCAACGTTTTGTTTTATTAGGGTTCAAATGTATA4850.11243899894515992No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4830.11197533297012832No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT4790.11104800102006514No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4770.11058433504503357No Hit
CAGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT4760.11035250205751776No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4740.10988883608248619No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4740.10988883608248619No Hit
GTTTTAGTACAATATGTTCCTGTGGTAGAGTACCCCAACTTCCAATGACA4730.10965700309497038No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4700.108961504132423No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT4700.108961504132423No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4680.1084978381573914No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4620.10710684023229665No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4550.1054840093196861No Hit
CCCTAATAAAACAAAACGTTGGGGCTACTCTCTTAACTTCATGGGATATG4520.10478851035713872No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4510.10455667736962292No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4490.10409301139459133No Hit
GGATATGTCATTGGAAGTTGGGGTACTCTACCACAGGAACATATTGTACT4480.10386117840707554No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4450.10316567944452816No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4450.10316567944452816No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4390.1017746815194334No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4380.1015428485319176No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4360.10107918255688603No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4360.10107918255688603No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4350.10084734956937022No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4340.10061551658185443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)