FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431345
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG22840.5295065434860726No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAAAG13750.31877035783421626No Hit
GTGTAAATGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13530.3136700321088688No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12960.3004555518204685No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10920.25316162236724665No Hit
CTCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACTCTACCACAG10660.247133964691836No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10600.24574296676674123No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAT10450.24226547195400433No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10370.240410808053878No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10260.23786064519120426No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA9410.2181548412523618No Hit
CCCTCATAAAACAAAACGTTGGGGCTACTCTCTTAACTTCATGGGATATG8790.2037811960263826No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8750.20285386407631942No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCTTAT8570.19868087030103515No Hit
ATATGTTCCTGTGGTAGAGTACCCCAACTTCCAATGACATATCCCATGAA8420.19520337548829822No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8360.19381237756320346No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA8100.18778471988779286No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7880.1826843941624454No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7860.1822207281874138No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7860.1822207281874138No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTAAGGTT7810.18106156324983483No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT7460.17294740868678204No Hit
GTACAATATGTTCCTGTGGTAGAGTACCCCAACTTCCAATGACATATCCC7140.16552875308627663No Hit
GTGGTAGAGTACCCCAACTTCCAATGACATATCCCATGAAGTTAAGAGAG7010.16251492424857134No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAA6740.1562554335856449No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6680.15486443566055014No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6620.15347343773545538No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6500.15069144188526584No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6410.14860494499762372No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAATGGGGCAGCA6370.14767761304756052No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6240.1446637842098552No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA6200.14373645225979204No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6130.14211362134718147No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5910.137013295621834No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC5900.13678146263431823No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGT5860.13585413068425506No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCA5820.1349267987341919No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5820.1349267987341919No Hit
CATTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5730.13284030184654974No Hit
CTCTACCACAGGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAA5610.1300583059963602No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA5540.12843547508374967No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5510.1277399761212023No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5500.1275081431336865No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT5380.12472614728349697No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5380.12472614728349697No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5330.123566982345918No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5300.12287148338337062No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5300.12287148338337062No Hit
GTTTTAGTACAATATGTTCCTGTGGTAGAGTACCCCAACTTCCAATGACA5280.12240781740833903No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATATGTT5270.12217598442082324No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5250.12171231844579164No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5250.12171231844579164No Hit
CATTTGAACCCTAATAAAACAAAACGTTGGGGCTACTCTCTTAACTTCAT5160.1196258215581495No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5160.1196258215581495No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5150.11939398857063373No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5110.11846665662057054No Hit
CCCTAATAAAACAAAACGTTGGGGCTACTCTCTTAACTTCATGGGATATG5040.11684382570796No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5000.11591649375789681No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4980.11545282778286521No Hit
GTTTTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC4920.11406182985777046No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4910.11382999687025468No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4900.11359816388273887No Hit
AGTTAAGAGAGTAGCCCCAACGTTTTGTTTTATTAGGGTTCAAATGTATA4870.11290266492019149No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4840.11220716595764411No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT4780.11081616803254934No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4770.11058433504503357No Hit
CAGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT4740.10988883608248619No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4720.10942517010745459No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4710.10919333711993878No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTA4690.10872967114490721No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4670.10826600516987563No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4580.10617950828223349No Hit
GGATATGTCATTGGAAGTTGGGGTACTCTACCACAGGAACATATTGTACT4480.10386117840707554No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4410.10223834749446498No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4410.10223834749446498No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4340.10061551658185443No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT4320.10015185060682284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)