FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607402
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG30750.5062545068998785No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28970.47694936796388554No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23250.3827777978999082No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT21910.3607166258919134No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA17310.2849842443719316No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT16460.27099021735193496No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16180.26638042021593605No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA15850.26094744501993733No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15700.25847791083993793No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGACTAATTATGCCTGCTAGG15650.25765473277993817No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14730.2425082564759418No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13890.2286788650679451No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13780.22686787333594557No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA13140.21633119416794808No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT12130.19970299735595207No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12020.1978920056239525No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC11740.1932822084879536No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11570.19048340308395428No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT11550.19015413185995436No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10330.1700685871959592No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9970.1641417051639606No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9760.16068435731196146No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG9370.154263568443963No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA9250.15228794109996344No Hit
CCCTAGCAGCCATGGGAACGAGGTGTATTTCCGAGAGAGGACAACCGAGT9230.15195866987596354No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9140.15047694936796388No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC9130.15031231375596393No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG9110.149983042531964No Hit
GATTAAGACTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT8870.14603178784396495No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8820.14520860978396516No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8660.14257443999196578No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT8650.14240980437996584No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG8560.14092808387196618No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC8520.14026954142396633No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8490.13977563458796646No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA8460.13928172775196657No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8390.13812927846796685No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8390.13812927846796685No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8320.13697682918396714No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA8250.1358243798999674No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8210.13516583745196756No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8150.1341780237799678No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT7930.13055604031596868No Hit
GCCTACGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7920.1303914047039687No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7900.13006213347996878No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7890.12989749786796884No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAGTCTTAATCTCCTTTTTTCA7550.12429988705997017No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7300.12018399675997116No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT7250.11936081869997135No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCTTG7250.11936081869997135No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7130.11738519135597182No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7110.1170559201319719No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG7060.11623274207197211No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6990.11508029278797238No Hit
CCTACGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCA6940.11425711472797258No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6930.11409247911597263No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT6810.11211685177197309No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6810.11211685177197309No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA6760.11129367371197328No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6750.11112903809997333No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6740.11096440248797336No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6660.10964731759197369No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6650.10948268197997374No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6650.10948268197997374No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6630.10915341075597379No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACA6610.10882413953197387No Hit
CAAGCTTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTA6520.10734241902397425No Hit
GGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGA6500.10701314779997431No Hit
GATTAAAACTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT6440.10602533412797455No Hit
GGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTT6380.1050375204559748No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6320.10404970678397503No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT6310.10388507117197507No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6290.10355579994797515No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT6260.10306189311197525No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6190.10190944382797555No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6170.10158017260397563No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6160.10141553699197565No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA6150.10125090137997571No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA6090.10026308770797593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)