FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607402
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG35780.5890662197358587No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28900.4757969186798858No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT24170.39792427420390447No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT20560.3384908182719188No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16800.2765878281599336No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16620.27362438714393433No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA16560.2726365734719346No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGACTAATTATGCCTGCTAGG15550.25600837665993853No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA15470.25469129176393884No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT15060.2479412316719405No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14550.2395448154599425No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA13400.22061172007994703No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13150.21649582977994802No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12860.2117213970319492No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT12520.2061237862239505No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT12450.20497133693995082No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11940.19657492072795282No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC11820.1945992933839533No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11620.19130658114395407No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10160.16726978179195984No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9310.1532757547719632No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA9170.15097085620396378No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9140.15047694936796388No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT9140.15047694936796388No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8850.14570251661996503No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA8720.14356225366396552No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8680.1429037112159657No Hit
GATTAAGACTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT8590.14142199070796604No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8530.14043417703596628No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG8490.13977563458796646No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT8460.13928172775196657No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8430.1387878209159667No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8340.13730610040796704No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8030.13220239643596826No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8010.13187312521196834No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC7990.13154385398796842No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA7910.13022676909196876No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAGTCTTAATCTCCTTTTTTCA7890.12989749786796884No Hit
GCCTACGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7880.12973286225596886No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7880.12973286225596886No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG7850.12923895541996896No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7780.12808650613596928No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7740.1274279636879694No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7710.12693405685196954No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT7690.12660478562796962No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC7580.12479379389597005No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7470.12298280216397049No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7350.12100717481997095No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7310.12034863237197112No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7290.1200193611479712No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7260.11952545431197133No Hit
CCCTAGCAGCCATGGGAACGAGGTGTATTTCCGAGAGAGGACAACCGAGT7240.11919618308797138No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7230.11903154747597143No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7200.11853764063997155No Hit
GGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGA7200.11853764063997155No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG7190.1183730050279716No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7100.11689128451997195No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA6910.11376320789197271No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6840.11261075860797298No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6760.11129367371197328No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACA6690.11014122442797357No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCTTG6630.10915341075597379No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6510.10717778341197427No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6480.10668387657597439No Hit
GTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6420.10569606290397462No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG6420.10569606290397462No Hit
GTCTGCACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT6400.10536679167997472No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6360.10470824923197487No Hit
GATTAAAACTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT6340.10437897800797495No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG6290.10355579994797515No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6240.10273262188797534No Hit
GTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6230.10256798627597538No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6150.10125090137997571No Hit
CAAGCTTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTA6140.10108626576797575No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA6120.10075699454397583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)