FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572440
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG32920.5750821046747258No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT27170.47463489623366645No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT24870.43445601285724267No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23490.4103486828313884No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG20700.3616099503878136No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16340.2854447627698973No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC15320.2676263014464398No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG14440.2522535112850255No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA13930.24334428062329677No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA13840.24177206344769755No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA13100.22884494444832645No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG11930.20840612116553697No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11750.20526168681433862No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11570.20211725246314025No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA10770.18814198868003634No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10220.17853399482915241No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9390.1640346586541821No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9220.16106491510027252No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGAAAACTGCCTGTAA9040.15792048074907414No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8930.15599888197889736No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8850.15460135560058696No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACC8760.15302913842498778No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTTAGTTTTTGTACAATA8370.14621619733072463No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8350.14586681573614702No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7970.13922856543917267No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7840.13695758507441827No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7770.1357347494933967No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7630.1332890783313535No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7610.13293969673677591No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7600.13276500593948712No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7530.13154217035846552No Hit
CCTAATACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTC7530.13154217035846552No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTTAGTTTTTGT7520.13136747956117673No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTTAGTTTTTGTACAAT7450.13014464398015513No Hit
ATACCTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGC7420.1296205715882887No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7340.12822304520997835No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7150.12490392006149116No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA7140.12472922926420235No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7000.12228355810215917No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6990.12210886730487037No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTTAGTTTTTGTACAATATGTT6830.11931381454824959No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA6640.11599468939976242No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6560.11459716302145204No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTCCTG6500.11354901823771923No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT6220.10865767591363287No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA6150.10743484033261129No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6140.10726014953532248No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6110.10673607714345608No Hit
GGCTAAGGCCCACTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGA6080.10621200475158968No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5950.10394102438683529No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5910.10324226119768011No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5860.1023688072112361No Hit
GATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT5770.10079659003563693No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGTGTGTAGTTTCTCTCTTATA5760.10062189923834813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)