FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483503
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC27830.5755910511413579No Hit
CCTCCTGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20860.43143475841928597No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAACCTTAT17370.3592532000835569No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15310.316647466510032No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15170.3137519312186274No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14780.3056857971925717No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA14340.2965855434195858No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13840.28624434595028364No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG12450.2574958169856237No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12310.25460028169421905No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCCTCTGACTTCTTTCCTTCTATTCG12110.2504638027064982No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11760.24322496447798672No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAA11180.23122917541359617No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10870.22481763298262883No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG10730.22192209769122426No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10640.22006068214674984No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10580.21881973845043362No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC10230.2115809002219221No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10120.20930583677867565No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10000.20682394938604312No Hit
GCCATGGGAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGTTGTCAGTC9630.1991714632587595No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9600.1985509914106014No Hit
ATGCAGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC9240.19110532923270382No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9000.1861415544474388No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8980.18572790654866672No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8960.18531425864989462No Hit
GTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTTCCTGTGGTAAA8880.1836596670548063No Hit
CAGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8590.17766177252261103No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8510.17600718092752268No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8400.1737321174842762No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG8340.17249117378795994No Hit
GCATAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCCTATGGCCAAG8320.17207752588918784No Hit
TTCGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGA8280.17125023009164367No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8260.17083658219287162No Hit
CTTATATAAAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT8120.167941046901467No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8090.16732057505330886No Hit
TCCTAGTACAAAGACCTTTAACCTGATCTCCTCCCCTAACTCCTCCCAGT7910.16359774396436008No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7810.16152950447049969No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7750.1602885607741834No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG7650.15822032128032298No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG7650.15822032128032298No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7640.15801349733093692No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA7640.15801349733093692No Hit
GGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC7590.1569793775840067No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7550.15615208178646256No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7470.1544974901913742No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT7390.15284289859628586No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT7270.15036101120365333No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAAC7180.14849959565917895No Hit
ACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC7040.14560406036777435No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7040.14560406036777435No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6980.1443631166714581No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6940.14353582087391392No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG6910.14291534902575578No Hit
GGATAATAAGGTTTAATTCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6890.1425017011269837No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6890.1425017011269837No Hit
CACAAAATGAGGCGCTGCGAGTAGTTTCTCTCTTATATAAAATGCCAGCC6870.1420880532282116No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6870.1420880532282116No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTGGTTGATGTTCCTGG6760.13981298978496512No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6710.13877887003803493No Hit
CCCCTAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA6670.13795157424049076No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6650.13753792634171866No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6640.13733110239233262No Hit
GGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA6630.13712427844294658No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6630.13712427844294658No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6570.1358833347466303No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6540.1352628628984722No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6470.13381509525276988No Hit
GTATGGATCGGCATAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6460.13360827130338385No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6430.1329877994552257No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6400.1323673276070676No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6390.13216050365768153No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6380.1319536797082955No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA6370.13174685575890946No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC6320.13071273601197925No Hit
CATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGG6320.13071273601197925No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6210.12843767256873279No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6140.12698990492303047No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6130.12678308097364444No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6100.1261626091254863No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6040.12492166542917003No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6010.12430119358101191No Hit
ATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC5970.12347389778346773No Hit
CTTTAGGGCATGGACATTGACGCGTATAAAGAATTTGGAGCTTCTGTGGA5950.12306024988469565No Hit
CTCCTATGCCGATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGC5930.12264660198592356No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5910.12223295408715147No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA5850.12099201039083521No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5840.12078518644144919No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5760.11913059484636083No Hit
CCTTTAACCTGATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACAAACAG5760.11913059484636083No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5440.11251222846600745No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5430.11230540451662141No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5430.11230540451662141No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5400.11168493266846327No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5350.11065081292153307No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5320.11003034107337492No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5300.10961669317460285No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5290.10940986922521681No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5250.10858257342767264No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT5240.10837574947828658No Hit
CTCCATGTTCGGTGCAGGGTCCCCAATCTTCGAGAAGATTGACGATATGG5240.10837574947828658No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5230.10816892552890055No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5220.1079621015795145No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5090.10527339023749593No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5090.10527339023749593No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5010.1036187986424076No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5010.1036187986424076No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG5010.1036187986424076No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCCTACAGACCACCAAATGCC5010.1036187986424076No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5000.10341197469302156No Hit
GACGTGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4970.10279150284486342No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4970.10279150284486342No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4930.10196420704731925No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4930.10196420704731925No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4930.10196420704731925No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4870.10072326335100298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)