FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483503
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC25560.5286420146307261No Hit
CCTCCTGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22770.4709381327520201No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16710.34560281942407806No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAACCTTAT16010.33112514296705503No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15930.3294705513719667No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14510.30010155055914856No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14350.29679236736897185No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA12760.263907359416591No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12610.2608050001758004No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG12210.25253204220035863No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAA12010.24839556321263775No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11750.24301814052860066No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11540.23867483759149374No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG11290.23350423885684268No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11130.23019505566666598No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10790.2231630413875405No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCCTCTGACTTCTTTCCTTCTATTCG10510.21737197080473133No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10210.21116725232315No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10100.20889218887990355No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9930.20537618174034078No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC9900.20475570989218267No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9490.1962759279673549No Hit
GTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTTCCTGTGGTAAA9330.19296674477717823No Hit
ATGCAGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC9320.19275992082779217No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG8960.18531425864989462No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8930.18469378680173648No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8650.1789027162189273No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8630.1784890683201552No Hit
CAGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8310.17187070193980183No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8090.16732057505330886No Hit
TTCGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGA8030.1660796313569926No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG7970.16483868766067636No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7950.16442503976190426No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7900.16339092001497404No Hit
GCATAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCCTATGGCCAAG7890.163184096065588No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7880.16297727211620197No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7860.16256362421742987No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAAC7830.16194315236927176No Hit
TCCTAGTACAAAGACCTTTAACCTGATCTCCTCCCCTAACTCCTCCCAGT7780.16090903262234155No Hit
GCCATGGGAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGTTGTCAGTC7690.15904761707786716No Hit
CTTATATAAAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT7510.15532478598891838No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7200.14891324355795105No Hit
GGATAATAAGGTTTAATTCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7170.1482927717097929No Hit
CTCCTATGCCGATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGC7130.14746547591224873No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6910.14291534902575578No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT6850.14167440532943953No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6850.14167440532943953No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6770.14001981373435118No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6770.14001981373435118No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA6740.13939934188619305No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6710.13877887003803493No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6640.13733110239233262No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6620.13691745449356055No Hit
ACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC6530.13505603894908613No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6530.13505603894908613No Hit
GGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC6490.13422874315154196No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG6450.1334014473539978No Hit
GTATGGATCGGCATAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6370.13174685575890946No Hit
CCCCTAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA6320.13071273601197925No Hit
CTTTAGGGCATGGACATTGACGCGTATAAAGAATTTGGAGCTTCTGTGGA6290.13009226416382114No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6270.12967861626504903No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG6220.12864449651811882No Hit
CCTTTAACCTGATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACAAACAG6200.12823084861934672No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6190.12802402466996068No Hit
GGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA6140.12698990492303047No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6090.12595578517610026No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6060.12533531332794212No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6050.12512848937855608No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5990.12388754568223982No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5950.12306024988469565No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5940.1228534259353096No Hit
CACAAAATGAGGCGCTGCGAGTAGTTTCTCTCTTATATAAAATGCCAGCC5930.12264660198592356No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5900.12202613013776545No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5830.12057836249206313No Hit
CATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGG5750.11892377089697478No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5740.11871694694758875No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5730.1185101229982027No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5710.11809647509943061No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5680.11747600325127248No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5660.1170623553525004No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5590.11561458770679808No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5520.1141668200610958No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5510.11395999611170976No Hit
GTATTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT5500.11375317216232371No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5470.11313270031416559No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTGGTTGATGTTCCTGG5450.1127190524153935No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5440.11251222846600745No Hit
ATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC5430.11230540451662141No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5410.11189175661784932No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5400.11168493266846327No Hit
CTCCATGTTCGGTGCAGGGTCCCCAATCTTCGAGAAGATTGACGATATGG5390.11147810871907723No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5380.11127128476969118No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTGATCTCCTCC5300.10961669317460285No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5290.10940986922521681No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5260.10878939737705867No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5260.10878939737705867No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5240.10837574947828658No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5190.10734162973135637No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5160.10672115788319823No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCCTACAGACCACCAAATGCC5150.1065143339338122No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5150.1065143339338122No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5130.10610068603504011No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5130.10610068603504011No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5070.10485974233872386No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5050.10444609443995177No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAATACAATA5040.10423927049056574No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATTCC5010.1036187986424076No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT5000.10341197469302156No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4940.1021710309967053No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4940.1021710309967053No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA4900.10134373519916112No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4880.10093008730038903No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4880.10093008730038903No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4860.10051643940161695No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT4840.10010279150284487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)