FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287639
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13450.4676000125156881No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT13380.4651664065025952No Hit
GTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCATGTTCAGG11690.40641220418649765No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8980.31219688567961923No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8570.29794290760293285No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC7950.276388111486968No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7330.25483331537100323No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG7030.24440357531489124No Hit
ATCCTAACCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6620.23014959723820483No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6360.22111048918957446No Hit
GTGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC6060.21068074913346246No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5880.20442290509979522No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5730.19920803507173923No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5380.18704000500627524No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT5310.18460639899318243No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5270.18321576698570083No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5200.18078216097260802No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTGAGGTT5110.17765323895577442No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTCGTTT5070.17626260694829282No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4940.17174305292397762No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4870.16930944691088481No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4860.16896178890901442No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4810.16722349889966243No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4690.16305160287721762No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4680.16270394487534723No Hit
CTTGATTTTCAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAA4670.1623562868734768No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4520.15714141684542082No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4350.15123123081362402No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4280.14879762480053121No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4210.14636401878743843No Hit
TCCACAGAGTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCA4200.14601636078556804No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4190.14566870278369762No Hit
CCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC4130.14358275477247523No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4110.14288743876873441No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4110.14288743876873441No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4110.14288743876873441No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAGAGTCCAA4100.14253978076686402No Hit
GTTTTATCCTAACCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4040.1404538327556416No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG4000.13906320074816No Hit
GAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTC3950.137324910738808No Hit
GAGTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCATGTTCA3940.13697725273693762No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3900.13558662072945601No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA3900.13558662072945601No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGTAGATCTCGAATAGAAGGAAA3880.13489130472571523No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA3880.13489130472571523No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGTAGATCTCGAA3830.13315301471636323No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3730.12967643469765922No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3710.12898111869391843No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3700.128633460692048No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3650.126895170682696No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGTAATGTCAACGACCGACCT3590.12480922267147361No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATC3530.12272327466025122No Hit
GAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATT3530.12272327466025122No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3440.11959435264341761No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3370.11716074663032483No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3340.11611777262471362No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3330.11577011462284323No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3320.11542245662097281No Hit
ATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACTTG3270.11368416661162081No Hit
ATACAGCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTG3270.11368416661162081No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA3230.11229353460413923No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3230.11229353460413923No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3210.11159821860039841No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3170.11020758659291681No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC3160.10985992859104642No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3160.10985992859104642No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3160.10985992859104642No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3140.1091646125873056No Hit
GAGTGATTCATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGG3100.10777398057982401No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3070.10673100657421282No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3060.10638334857234241No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3040.10568803256860163No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTCGT3030.10534037456673123No Hit
TTTCCTGTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTT3030.10534037456673123No Hit
CCCCAACACAGAATGGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATG2980.10360208455737921No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACGAGGCATTATT2950.102559110551768No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2940.10221145254989761No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG2940.10221145254989761No Hit
ATACAGAGCAGAGGCGGTGTCGAGTAGATCTCGAATAGAAGGAAAGAAGT2910.1011684785442864No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG2900.100820820542416No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG2890.10047316254054561No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC2880.10012550453867522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)