FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287639
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13300.4623851424876321No Hit
GTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCATGTTCAGG12700.4415256623754081No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT10490.36469324396204966No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9100.31636878170206406No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7860.27325918947013444No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG7410.25761457938596644No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC7400.257266921384096No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6980.24266528530553924No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6630.23049725524007525No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6260.21763390917087042No Hit
ATCCTAACCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6080.21137606513720325No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6080.21137606513720325No Hit
GTGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC5660.19677442905864642No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT5600.19468848104742403No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5350.18599703100066403No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5150.17904387096325602No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4900.17035242091649602No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4880.16965710491275524No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4810.16722349889966243No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4780.16618052489405122No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTGAGGTT4480.15575078483793922No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTCGTTT4420.1536648368267168No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4370.1519265468173648No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4340.1508835728117536No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4260.14810230879679043No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG4250.14775465079492003No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4170.14497338677995683No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4100.14253978076686402No Hit
GAGTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCATGTTCA4080.1418444647631232No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4060.14114914875938242No Hit
GTTTTATCCTAACCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4020.13975851675190082No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3990.1387155427462896No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGTAATGTCAACGACCGACCT3980.13836788474441922No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3890.13523896272758562No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3820.13280535671449284No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3800.13211004071075202No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGTAGATCTCGAA3750.13037175070140003No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA3700.128633460692048No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3700.128633460692048No Hit
CTTGATTTTCAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAA3650.126895170682696No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3640.12654751268082562No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3610.1255045386752144No Hit
TCCACAGAGTATGTAAATAATGCCTCGTTTTGAAGTAATGATTAACTGCA3600.12515688067334402No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3500.12168030065464001No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAGAGTCCAA3470.1206373266490288No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA3450.11994201064528802No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG3430.11924669464154722No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3390.11785606263406562No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3360.11681308862845441No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3260.11333650860975042No Hit
GAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATT3260.11333650860975042No Hit
GAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTC3200.11125056059852802No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3200.11125056059852802No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3190.11090290259665762No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3190.11090290259665762No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3160.10985992859104642No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3160.10985992859104642No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATC3160.10985992859104642No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3140.1091646125873056No Hit
CCCCAACACAGAATGGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATG3080.10707866457608321No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC3010.10464505856299042No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA3010.10464505856299042No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACGAGGCATTATT3010.10464505856299042No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTCGT3010.10464505856299042No Hit
ATATTGTACTGAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA2980.10360208455737921No Hit
ATACAGCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTG2930.10186379454802721No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2920.10151613654615682No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC2910.1011684785442864No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2910.1011684785442864No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGTAGATCTCGAATAGAAGGAAA2880.10012550453867522No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG2880.10012550453867522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)