FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451219
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55051.2200284119241434No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG24800.5496222455171436No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17780.3940436905360812No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA17110.37919502503219055No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG15280.338638222237982No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT14780.32755712857836217No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA13410.2971949319510038No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11920.26417327284533676No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT11680.2588543478887192No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA11440.25353542293210174No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10450.23159485748605446No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10400.23048674812009246No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10290.2280489075149761No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10190.22583268878305215No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC10130.22450295754389776No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA10020.2220651169387814No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG9960.220735385699627No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC9890.21918403258728023No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9720.2154164607430095No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA9630.21342186388427792No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCACTATTACCAATTTTCTT9480.21009753578639195No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA9350.2072164514348908No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9220.20433536708338967No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGT9180.20344887959062008No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT9170.20322725771742767No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9120.2021191483514657No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9090.2014542827318885No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA9020.19990292961954173No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA8500.18837859221353712No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8220.18217317976415No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA8180.18128669227138042No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8120.17995696103222603No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC7960.1764110110611477No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA7910.17530290169518573No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA7880.17463803607560852No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT7820.17330830483645415No Hit
ACTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAA7800.17286506109006936No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7530.16688127051387464No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7430.16466505178195065No Hit
GCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGGCC7370.1633353205427963No Hit
GTACAACATCTTGAGTCCCTTTTTACCACTATTACCAATTTTCTTTTGTC7280.1613407236840647No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG7280.1613407236840647No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT7210.15978937057171796No Hit
GGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTT7010.15535693310787002No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6950.15402720186871563No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6910.15314071437594606No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACAT6910.15314071437594606No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6890.15269747062956124No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6780.1502596300244449No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT6770.1500380081512525No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6710.14870827691209812No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6630.14693530192655893No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6590.14604881443378936No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT6530.14471908319463497No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG6530.14471908319463497No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCT6530.14471908319463497No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6520.1444974613214426No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6450.1429461082090958No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTTT6410.14205962071632622No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC6390.14161637696994142No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGTGGTAAAAA6350.14072988947717185No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTTTGG6340.14050826760397944No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6260.13873529261844028No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6260.13873529261844028No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT6180.1369623176329011No Hit
GGTAACAGTGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCA6180.1369623176329011No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6180.1369623176329011No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5940.13164339267628358No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5940.13164339267628358No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5830.1292055520711672No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT5830.1292055520711672No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5780.12809744270520523No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG5740.12721095521243564No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG5690.12610284584647366No Hit
CAAGCTTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTA5690.12610284584647366No Hit
ACCTAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5660.12543798022689648No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC5630.12477311460731928No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG5500.12189203025581813No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT5490.12167040838262572No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5480.12144878650943335No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG5440.12056229901666374No Hit
GGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCC5410.11989743339708656No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5360.11878932403112458No Hit
CAATTACATATCCCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5350.11856770215793219No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT5330.11812445841154738No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA5290.1172379709187778No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA5210.11546499593323863No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5210.11546499593323863No Hit
GCCCAAAAGCCCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5050.11191904596216029No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC4990.1105893147230059No Hit
GGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCA4990.1105893147230059No Hit
CACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAA4940.10948120535704392No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC4850.10748660849831235No Hit
ACCCTGCACCGAACATGGAGAACACAACATCAGGATTCCTAGGACCCCTG4810.10660012100554277No Hit
ATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGGCCGTTGC4800.10637849913235037No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4760.10549201163958077No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTTTGGGCTTT4740.10504876789319598No Hit
GAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTG4720.1046055241468112No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4710.1043839022736188No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCACTAT4690.103940658527234No Hit
CATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAA4670.10349741478084921No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4620.10238930541488724No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCAC4580.10150281792211764No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATGTAA4530.10039470855615566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)