FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451219
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54701.2122716463624095No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG27180.602368251336934No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17250.38229773125688415No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA17030.3774220500466514No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG15570.34506525656056153No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT14760.32711388483197734No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA13960.3093841349765856No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC11440.25353542293210174No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT11220.24865974172186897No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA11080.24555703549717545No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10920.2420110855260971No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10880.24112459803332748No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10800.23935162304778834No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA10780.23890837930140352No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG10720.23757864806224915No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA10430.23115161373966964No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG10330.22893539500774568No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA10060.22295160443155096No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9950.22051376382643462No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9950.22051376382643462No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC9750.2160813263625867No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA9720.2154164607430095No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA9720.2154164607430095No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9600.21275699826470074No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT9320.20655158581531363No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCACTATTACCAATTTTCTT9270.20544347644935165No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGT9220.20433536708338967No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8980.1990164421267721No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8640.19148129843823067No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA8610.1908164328186535No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT8320.18438939849607397No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA8230.1823948016373424No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA8140.18040020477861082No Hit
ACTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAA7950.1761893891879553No Hit
GTACAACATCTTGAGTCCCTTTTTACCACTATTACCAATTTTCTTTTGTC7710.17087046423133778No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7690.17042722048495298No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7630.1690974892457986No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7610.16865424549941382No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC7590.16821100175302903No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG7560.16754613613345185No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7540.16710289238706702No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7500.16621640489429745No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTTTGG7420.16444342990875827No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT7300.1617839674304495No Hit
GCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGGCC7300.1617839674304495No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7170.15890288307894837No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG7110.15757315183979398No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGTGGTAAAAA6900.15291909250275365No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6890.15269747062956124No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6870.15225422688317647No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6820.1511461175172145No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACAT6810.15092449564402208No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6790.15048125189763728No Hit
GGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTT6750.1495947644048677No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6700.14848665503890573No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTTT6610.14649205818017416No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6600.14627043630698175No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6600.14627043630698175No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT6590.14604881443378936No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT6560.14538394881421218No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC6410.14205962071632622No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6340.14050826760397944No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG6280.13917853636482505No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCT6210.1376271832524783No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT6170.1367406957597087No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC6000.13297312391543797No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5960.13208663642266838No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG5920.13120014892989879No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT5870.1300920395639368No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5850.129648795817552No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5830.1292055520711672No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG5820.12898393019797483No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC5790.12831906457839762No Hit
ACCTAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5740.12721095521243564No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC5720.12676771146605087No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT5720.12676771146605087No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA5710.12654608959285846No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5680.12588122397328125No Hit
CAAGCTTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTA5640.12499473648051168No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTTTGGGCTTT5590.1238866271145497No Hit
GGTAACAGTGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCA5540.12277851774858772No Hit
ATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGGCCGTTGC5530.12255689587539532No Hit
ACCCTGCACCGAACATGGAGAACACAACATCAGGATTCCTAGGACCCCTG5430.12034067714347135No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5410.11989743339708656No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG5400.11967581152389417No Hit
GGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCC5400.11967581152389417No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5340.11834608028473978No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC5230.11590823967962342No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5160.11435688656727665No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5130.11369202094769947No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCACTAT5130.11369202094769947No Hit
CAATTACATATCCCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5010.1110325584693907No Hit
CCCCAACCTCCAATCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGC4980.1103676928498135No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCTTGTCCTCCAATTTGTCCTG4970.11014607097662112No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCAC4960.10992444910342872No Hit
GCCCAAAAGCCCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4950.1097028272302363No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4920.10903796161065914No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT4840.10726498662511996No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT4800.10637849913235037No Hit
GTGATTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA4790.10615687725915796No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA4750.10527038976638839No Hit
GGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCA4720.1046055241468112No Hit
CACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAA4710.1043839022736188No Hit
CCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAA4640.10283254916127202No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATGTAA4620.10238930541488724No Hit
CATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAA4600.10194606166850242No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4590.10172443979531004No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4580.10150281792211764No Hit
AACATGGAGAACACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4580.10150281792211764No Hit
CATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT4540.10061633042934806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)