FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381680
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27780.7278348354642633No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15580.40819534688744497No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10370.27169356529029554No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG10220.26776357157828545No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9870.25859358625026196No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9650.2528295954726473No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9250.24234961224062043No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9230.2418256130790191No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9070.2376336197862083No Hit
CACTTACAGTTAATGAAAAGAGAAGACTGCAATTGATTATGCCTGCTAGG8910.23344162649339759No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8730.22872563403898552No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8350.21876964996856005No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8240.21588765457975267No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8150.21352965835254664No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7570.19833368266610774No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7380.193355690630895No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7040.1844477048836722No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6960.18235170823726682No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6910.18104171033326347No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6880.18025571159086146No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6840.17920771326765877No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6830.1789457136868581No Hit
CATTTGCACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT6740.17658771745965207No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG6660.17449172081324668No Hit
CCTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTGTATGCATGTAT6620.17344372249004403No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6410.16794173129322992No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6410.16794173129322992No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT6230.16322573883881786No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6190.16217774051561518No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6160.16139174177321317No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5990.15693774889960177No Hit
ATATACATTTGCACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGC5970.1564137497380004No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT5950.1558897505763991No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5700.1493397610563823No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5650.14802976315237895No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC5580.14619576608677426No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5530.1448857681827709No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5520.14462376860197024No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5490.14383776985956823No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5430.1422657723747642No Hit
CTTACAGTTAATGAAAAGAGAAGACTGCAATTGATTATGCCTGCTAGGTT5370.14069377488996018No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5320.13938377698595683No Hit
ATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGTTAATGAAAAGAG5280.13833577866275412No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5240.13728778033955147No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5150.13492978411234544No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5020.1315237895619367No Hit
GAATACATGCATACAAGGGCATTAACGCAGGATAACCACATTGTGTAAAA4930.12916579333473066No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGT4850.1270697966883253No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4740.12418780129951792No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA4720.12366380213791658No Hit
GCCTAGCAGCCATGGAAACGAAGTATATTTGCGGGAGAGAACAACAGAGT4670.12235380423391323No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTTCACCAAACTCTGCAAG4640.12156780549151122No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTGCACC4640.12156780549151122No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG4580.1199958080067072No Hit
CATGCATACAAGGGCATTAACGCAGGATAACCACATTGTGTAAAAGGAGC4550.11920980926430517No Hit
AAAGAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA4550.11920980926430517No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTCTTTTCA4500.11789981136030181No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4480.1173758121987005No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4340.1137078180674911No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4260.11161182142108574No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA4260.11161182142108574No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4230.11082582267868371No Hit
AATATACATTTGCACCAAGACATTATCAAAAGATGTGAACAATTTGTAGG4180.10951582477468037No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4170.1092538251938797No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4170.1092538251938797No Hit
GGCATAATCAATTGCAGTCTTCTCTTTTCATTAACTGTAAGTGGGCCTAC4160.10899182561307902No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGGCCTCCGT4150.10872982603227835No Hit
GCTCTATATCGGGAAGCTTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4080.10689582896667366No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4080.10689582896667366No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4050.10610983022427165No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3980.10427583315866695No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG3970.10401383357786628No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTCTTTTCATTAAC3950.10348983441626494No Hit
CTAATATACATTTGCACCAAGACATTATCAAAAGATGTGAACAATTTGTA3930.10296583525466359No Hit
CTCTAAAGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA3930.10296583525466359No Hit
CAGTTAATGAAAAGAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTAT3920.10270383567386292No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3860.1011318381890589No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG3840.10060783902745755No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3840.10060783902745755No Hit
CTGTAAGTGGGCCTACAAATTGTTCACATCTTTTGATAATGTCTTGGTGC3820.10008383986585621No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCTTTGCCA3820.10008383986585621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)