FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381680
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA29770.7799727520435967No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15560.4076713477258437No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12180.31911548941521695No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC12010.3146614965416056No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11050.2895095367847411No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10130.26540557535107945No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9790.25649758960385666No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9420.2468036051142318No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9340.24470760846782647No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9200.24103961433661705No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9170.24025361559421504No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG9030.23658562146300569No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9020.236323621882205No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8990.23553762313980298No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8680.2274156361349822No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC8060.2111716621253406No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7900.20697966883252988No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7820.2048836721861245No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7470.19571368685810103No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7430.19466568853489835No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6940.18182770907566548No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6760.17711171662125338No Hit
CACTTACAGTTAATGAAAAGAGAAGACTGCAATTGATTATGCCTGCTAGG6690.17527771955564872No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6500.17029972752043596No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6430.16846573045483126No Hit
CCTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTGTATGCATGTAT6290.16479773632362188No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6270.16427373716202054No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT6070.1590337455460071No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5790.15169775728358834No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5750.15064975896038568No Hit
GGCATAATCAATTGCAGTCTTCTCTTTTCATTAACTGTAAGTGGGCCTAC5680.14881576189478096No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5610.14698176482917627No Hit
CATTTGCACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT5610.14698176482917627No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5530.1448857681827709No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5510.14436176902116957No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT5270.13807377908195348No Hit
ATATACATTTGCACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGC5220.13676378117795013No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5170.13545378327394678No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5160.1351917836931461No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5110.13388178578914273No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5110.13388178578914273No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5110.13388178578914273No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5080.13309578704674072No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5060.1325717878851394No Hit
ATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGTTAATGAAAAGAG4930.12916579333473066No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTCTTTTCA4880.1278557954307273No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGGCCTCCGT4850.1270697966883253No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTTCACCAAACTCTGCAAG4760.12471180046111927No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4760.12471180046111927No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA4720.12366380213791658No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4720.12366380213791658No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4720.12366380213791658No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTGCACC4690.12287780339551456No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4680.1226158038147139No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC4620.12104380632990987No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4460.11685181303709914No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTCTTTTCATTAAC4380.11475581639069378No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4370.11449381680989311No Hit
GCCTAGCAGCCATGGAAACGAAGTATATTTGCGGGAGAGAACAACAGAGT4370.11449381680989311No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4330.11344581848669041No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG4280.11213582058268708No Hit
GCTCTATATCGGGAAGCTTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4280.11213582058268708No Hit
CTGTAAGTGGGCCTACAAATTGTTCACATCTTTTGATAATGTCTTGGTGC4260.11161182142108574No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4240.11108782225948438No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4200.1100398239362817No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4170.1092538251938797No Hit
GAATACATGCATACAAGGGCATTAACGCAGGATAACCACATTGTGTAAAA4100.10741982812827498No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA4090.10715782854747431No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGT4080.10689582896667366No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4080.10689582896667366No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG4040.10584783064347097No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4040.10584783064347097No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4020.10532383148186962No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3970.10401383357786628No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3930.10296583525466359No Hit
ATATGAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC3930.10296583525466359No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT3910.10244183609306225No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG3910.10244183609306225No Hit
CTTACAGTTAATGAAAAGAGAAGACTGCAATTGATTATGCCTGCTAGGTT3900.10217983651226158No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTGCA3890.10191783693146092No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3850.10086983860825822No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3830.10034583944665688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)