FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200961
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8280.4120202427336648No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8130.4045561079015331No Hit
GGCACGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTG6360.3164793168823802No Hit
GATTTAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT6350.3159817078935714No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6340.31548409890476264No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5810.2891108224978976No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5550.2761729887888695No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5510.27418255283363435No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5470.2721921168783993No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5440.27069928991197295No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5440.27069928991197295No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5300.26373276406865015No Hit
ATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5240.2607471101357975No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5200.25875667418056236No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA5010.2493021033931957No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA4780.2378570966505939No Hit
ATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCTG4500.22392404496394824No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4350.21645991013181662No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4350.21645991013181662No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4300.21397186518777278No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4080.20302446743397973No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGACAAAGGCATTAAACCGTAT4070.20252685844517093No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4050.2015316404675534No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC3930.1955603326018481No Hit
CTTTAGGGCATGGACATTGACGCATATAAAGAATTTGGAGCTTCTGTGGA3920.19506272361303933No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT3860.1920770696801867No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC3790.1885938067585253No Hit
CATTTAAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTAACTTTAT3680.18312010788162877No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3460.17217271012783575No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3440.17117749215021819No Hit
TATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATG3440.17117749215021819No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA3360.16719662023974802No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACATTACCGCAG3320.16520618428451292No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3300.16421096630689536No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3290.1637133573180866No Hit
AATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTGG3280.16321574832927782No Hit
GTCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGTT3260.16222053035166029No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3240.16122531237404272No Hit
GCTTTAGGGCATGGACATTGACGCATATAAAGAATTTGGAGCTTCTGTGG3180.1582396584411901No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3150.15674683147476376No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3040.15127313259786723No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG3010.14978030563144093No Hit
CTCCAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA2960.14729226068739706No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG2940.1462970427097795No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG2930.14579943372097073No Hit
GGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTG2910.14480421574335317No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2910.14480421574335317No Hit
GCCTTGGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2900.1443066067545444No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG2870.1428137797881181No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA2820.1403257348440742No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2790.1388329078776479No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC2730.13584725394479524No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC2710.1348520359671777No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2710.1348520359671777No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA2700.13435442697836894No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA2680.13335920900075138No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2680.13335920900075138No Hit
GCTTTGGGGCATGGACATTGACGCATATAAAGAATTTGGAGCTTCTGTGG2630.1308711640567075No Hit
CCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATT2620.13037355506789874No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2620.13037355506789874No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2580.12838311911266365No Hit
GGTAACAGCGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCA2560.1273879011350461No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG2560.1273879011350461No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC2550.12689029214623734No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC2550.12689029214623734No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2540.12639268315742858No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2530.12589507416861978No Hit
GATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT2530.12589507416861978No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2530.12589507416861978No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2510.12489985619100223No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA2450.1219142022581496No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2440.1214165932693408No Hit
GGTCTAAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGT2420.12042137529172327No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2370.11793333034767939No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA2350.11693811237006185No Hit
CCTTCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA2340.11644050338125307No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2340.11644050338125307No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2320.11544528540363554No Hit
GCCTATAAGTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGA2310.11494767641482677No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA2290.1139524584372092No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATCTCC2260.11245963147078289No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG2260.11245963147078289No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA2250.11196202248197412No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACG2250.11196202248197412No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2230.11096680450435657No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2220.1104691955155478No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2210.10997158652673901No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATACTTGGTA2190.10897636854912146No Hit
AGTTAAGGGAGTAACCCCAGCGTTTGGTTTTATTAGGGTTTAAATGTATA2180.1084787595603127No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2180.1084787595603127No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2180.1084787595603127No Hit
TCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCT2170.10798115057150393No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2160.10748354158269516No Hit
TAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG2160.10748354158269516No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTA2130.10599071461626881No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2120.10549310562746005No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT2120.10549310562746005No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG2110.10499549663865128No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATACTTGGTAAGGTT2110.10499549663865128No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2110.10499549663865128No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT2100.10449788764984251No Hit
GGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTAAACAATATC2090.10400027866103373No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG2080.10350266967222496No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2080.10350266967222496No Hit
GTCTATTTACAGGCAGTTTACGAAAACATTGCTTGATTTTCAGTACAATA2050.10200984270579863No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT2050.10200984270579863No Hit
CATATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT2040.10151223371698986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)