FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200961
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7550.37569478655062427No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC7320.3642497798080224No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6650.33090997755783463No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6070.3020486562069257No Hit
GATTTAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT6050.3010534382293082No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG5850.2911012584531327No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5660.28164668766576595No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5470.2721921168783993No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5460.2716945078895905No Hit
GGCACGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTG5340.2657232000238852No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5280.2627375460910326No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5260.261742328113415No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5080.25278536631485715No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4830.24034514159463777No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA4720.23487144271774124No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4470.2224312179975219No Hit
ATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4460.22193360900871315No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4380.21795273709824292No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4350.21645991013181662No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4230.21048860226611132No Hit
CTTTAGGGCATGGACATTGACGCATATAAAGAATTTGGAGCTTCTGTGGA4220.20999099327730256No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4190.20849816631087625No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA4140.2060101213668324No Hit
CATTTAAACCCTAATAAAACCAAACGCTGGGGTTACTCCCTTAACTTTAT3990.1985459865347008No Hit
ATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCTG3960.19705315956827443No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC3740.1861057618144814No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3710.1846129348480551No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGACAAAGGCATTAAACCGTAT3710.1846129348480551No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3630.1806320629375849No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3620.18013445394877614No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC3470.17267031911664454No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3450.17167510113902695No Hit
GTCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGTT3330.1657037932733217No Hit
CTCCAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3200.15923487641880762No Hit
TATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATG3190.15873726742999886No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA3140.15624922248595496No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3140.15624922248595496No Hit
GATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT3060.1522683505754848No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3040.15127313259786723No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACATTACCGCAG3040.15127313259786723No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG2980.1482874786650146No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA2960.14729226068739706No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA2940.1462970427097795No Hit
GGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTG2900.1443066067545444No Hit
AATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTGG2900.1443066067545444No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG2880.14331138877692687No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA2850.14181856181050057No Hit
GCTTTAGGGCATGGACATTGACGCATATAAAGAATTTGGAGCTTCTGTGG2850.14181856181050057No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2820.1403257348440742No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC2790.1388329078776479No Hit
GCCTTGGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2770.13783768990003037No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG2730.13584725394479524No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2720.13534964495598648No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2710.1348520359671777No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG2690.13385681798956017No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2690.13385681798956017No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA2680.13335920900075138No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2660.13236399102313384No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC2610.12987594607908998No Hit
CCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATT2590.1288807281014724No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC2590.1288807281014724No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2570.12788551012385488No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC2560.1273879011350461No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2550.12689029214623734No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2550.12689029214623734No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2540.12639268315742858No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2530.12589507416861978No Hit
GCTTTGGGGCATGGACATTGACGCATATAAAGAATTTGGAGCTTCTGTGG2490.12390463821338468No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2470.12290942023576715No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2460.12241181124695838No Hit
GGTAACAGCGGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCA2430.12091898428053204No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2430.12091898428053204No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2420.12042137529172327No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG2400.11942615731410572No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2400.11942615731410572No Hit
CCTTCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA2400.11942615731410572No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG2360.11743572135887062No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2340.11644050338125307No Hit
GGTCTAAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGT2330.1159428943924443No Hit
GTCTATTTACAGGCAGTTTACGAAAACATTGCTTGATTTTCAGTACAATA2330.1159428943924443No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG2330.1159428943924443No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA2310.11494767641482677No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2300.11445006742601799No Hit
TAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG2280.11345484944840042No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2280.11345484944840042No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT2270.11295724045959166No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA2250.11196202248197412No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG2210.10997158652673901No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2180.1084787595603127No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG2170.10798115057150393No Hit
GCCTATAAGTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGA2150.10698593259388639No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC2150.10698593259388639No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2150.10698593259388639No Hit
TCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCT2140.10648832360507761No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT2130.10599071461626881No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG2120.10549310562746005No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATACTTGGTA2120.10549310562746005No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2100.10449788764984251No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA2090.10400027866103373No Hit
GGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTAAACAATATC2090.10400027866103373No Hit
GGGCACATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATC2070.1030050606834162No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2070.1030050606834162No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC2060.10250745169460743No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC2060.10250745169460743No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2060.10250745169460743No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2060.10250745169460743No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACG2060.10250745169460743No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTA2040.10151223371698986No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA2020.10051701573937231No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2020.10051701573937231No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGCCCACG2020.10051701573937231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)