FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285150
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT14970.5249868490268279No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10080.3534981588637559No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9930.34823776959495No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9900.34718569174118885No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8930.3131685078029107No Hit
GGCAAAGACCGGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGT7740.271436086270384No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7280.25530422584604595No Hit
AAACCTTATTATCCTGAACAGGCAGTTAATCATTACTTCAAAACTAGGCA6880.2412765211292302No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG6790.2381202875679467No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6480.22724881641241454No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCCTGTTCAGG6330.22198842714360864No Hit
TAGCCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCT6230.2184815009644047No Hit
GTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGCC6050.2121690338418376No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCTTGT5970.2093634928984745No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5900.2069086445730317No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5770.20234964054006663No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5660.19849202174294234No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5650.19814132912502191No Hit
TTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACC5580.19568648079957918No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC5550.194634402945818No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5530.1939330177099772No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5250.1841136244082062No Hit
GGATAACCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAAT5140.18025600561108188No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5060.17745046466771874No Hit
CCCAAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5050.17709977204979835No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5040.17674907943187795No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4810.16868314921970892No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4730.1658776082763458No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4690.1644748378046642No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4570.1602665263896195No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4550.1595651411537787No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAAG4480.15711029282833597No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACGTTAT4380.15360336664913202No Hit
GTGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC4290.15044713308784852No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4290.15044713308784852No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4260.14939505523408733No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4120.14448535858320183No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4070.14273189549359988No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4050.14203051025775906No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4020.1409784324039979No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4000.14027704716815712No Hit
GCCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT3990.13992635455023672No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3870.135718043135192No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3840.13466596528143082No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT3810.13361388742766966No Hit
TAACCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCT3710.1301069612484657No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3700.12975626863054535No Hit
GAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT3670.12870419077678413No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG3660.12835349815886377No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTCCACATTTCC3650.12800280554094337No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG3630.12730142030510258No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCGGGATGCTGGGTCTTCC3610.1266000350692618No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCAGACCTTTAACCT3610.1266000350692618No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG3600.1262493424513414No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3550.12449587936173945No Hit
GCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCTTTGACATACT3460.1213396458004559No Hit
GATAACCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATT3440.1206382605646151No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3430.12028756794669473No Hit
GTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG3390.11888479747501315No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3380.11853410485709276No Hit
ATCGACAATAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3380.11853410485709276No Hit
CTCCAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTCAA3340.11713133438541118No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3330.11678064176749078No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3310.11607925653165001No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3290.11537787129580922No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3270.11467648605996844No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3260.11432579344204805No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3220.11292302297036647No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3210.11257233035244608No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT3210.11257233035244608No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3210.11257233035244608No Hit
GGATAGCCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAAT3130.10976678940908295No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3130.10976678940908295No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3110.10906540417324215No Hit
GGCAGAGACCGGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGT3100.10871471155532175No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3060.1073119410836402No Hit
CAGCCTGTTTCGCTCGCAGCCGGTCTGGAGCGAACCTTATCGGGACTGAC3050.1069612484657198No Hit
AACAAGCTGCGAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAG3040.1066105558477994No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3030.106259863229879No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3030.106259863229879No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA3010.10555847799403822No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3010.10555847799403822No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2990.10485709275819743No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2980.10450640014027705No Hit
GGTCCTAGGAATCCTGATGTTGTGCTCTCCATGTTCGGTGCAGGGTCCCC2970.10415570752235666No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG2960.10380501490443626No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT2940.10310362966859549No Hit
CTCCTAGTACAATGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG2920.10240224443275468No Hit
CACCCAAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGA2910.1020515518148343No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2880.10099947396107312No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2860.10029808872523234No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC2860.10029808872523234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)