FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285150
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT13490.4730843415746098No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10090.35384885148167633No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9890.34683499912326843No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9290.3257934420480449No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9160.3212344380150798No Hit
GTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGCC7350.2577590741714887No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7320.25670699631772753No Hit
GGCAAAGACCGGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGT7090.2486410661055585No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCCTGTTCAGG6960.24408206207259336No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG6750.23671751709626512No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6250.21918288620024548No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6240.21883219358232509No Hit
AAACCTTATTATCCTGAACAGGCAGTTAATCATTACTTCAAAACTAGGCA6110.21427318954936No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCTTGT5840.20480448886550937No Hit
TTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACC5750.20164825530422584No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC5750.20164825530422584No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5730.20094687006838508No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5610.19673855865334033No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5450.19112747676661407No Hit
TAGCCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCT5450.19112747676661407No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5260.1844643170261266No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5190.18200946870068385No Hit
GTGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC5010.1756970015781168No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4970.17429423110643522No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4840.1697352270734701No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAAG4810.16868314921970892No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4700.1648255304225846No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4640.16272137471506226No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4590.16096791162546029No Hit
GGATAACCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAAT4550.1595651411537787No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACGTTAT4500.15781167806417673No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4410.15465544450289323No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4340.15220059617745046No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4300.1507978257057689No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4280.1500964404699281No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4260.14939505523408733No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4170.14623882167280378No Hit
GCCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4170.14623882167280378No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4140.14518674381904262No Hit
CCCAAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4090.14343328072944064No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3970.13922496931439593No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3860.1353673505172716No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT3850.13501665789935122No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3750.1315097317201473No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3660.12835349815886377No Hit
GCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCTTTGACATACT3650.12800280554094337No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3630.12730142030510258No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3550.12449587936173945No Hit
TAACCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCT3550.12449587936173945No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT3520.12344380150797825No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3500.12274241627213747No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTCCACATTTCC3480.12204103103629668No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3440.1206382605646151No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3440.1206382605646151No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG3420.11993687532877433No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3400.11923549009293355No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3280.11502717867788884No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3280.11502717867788884No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA3260.11432579344204805No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3230.11327371558828686No Hit
GTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG3220.11292302297036647No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCAGACCTTTAACCT3210.11257233035244608No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3170.11116955988076452No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG3090.10836401893740137No Hit
GAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT3080.10801332631948098No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3080.10801332631948098No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3040.1066105558477994No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3010.10555847799403822No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3000.10520778537611783No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATC3000.10520778537611783No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3000.10520778537611783No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2980.10450640014027705No Hit
GATAACCACACTGCGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATT2950.10345432228651585No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG2930.10275293705067508No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT2870.10064878134315274No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCGGGATGCTGGGTCTTCC2860.10029808872523234No Hit
ATCGACAATAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT2860.10029808872523234No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG2860.10029808872523234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)