FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421293
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24480.5810682826441456No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT12630.2997913566092957No Hit
GTATAATATGGTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC12170.28887258986026354No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11740.2786659165948639No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9720.2307182886969401No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9310.22098634442062887No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8990.21339068059521518No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8810.20911811969342003No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8310.19724989496621115No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATTCATTTGAACCCTAATAAAAC8030.1906036891189742No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGC7880.18704322170081156No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7590.18015965135903042No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7340.174225538995426No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA7030.1668672396645565No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCAAG6850.16259467876276132No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG6730.15974630482823118No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA6640.1576100243773336No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6610.15689793089370105No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6390.15167591201372918No Hit
GCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAA6360.15096381853009663No Hit
GCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTA6350.15072645403555246No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6200.1471659866173898No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6090.14455497717740384No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5990.14218133223196208No Hit
GAACAGACAACTCTGTTGTTCTCTCTCGGAAATACACCTCCTTTCCATGG5950.1412318742537854No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT5930.14075714526469701No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGAACAACAGAGT5840.1386208648137994No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5750.13648458436290184No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC5580.1324493879556508No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5550.1317372944720183No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGAATA5460.1296010140211207No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5260.12485372413023717No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC5250.12461635963569298No Hit
ATACTACAGAGTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGG5140.12200535019570703No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG5050.11986906974480943No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5040.11963170525026526No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG5030.11939434075572107No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4960.11773278929391184No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4950.11749542479936766No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4930.11702069581027931No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4910.11654596682119096No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4770.11322286389757247No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4620.10966239647940983No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACC4590.10895030299577729No Hit
GTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACCCG4590.10895030299577729No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC4570.10847557400668892No Hit
GGACATCCTTTGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCG4500.10681402254487969No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4480.10633929355579133No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4460.10586456456670298No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA4440.10538983557761464No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4380.10396564861034957No Hit
GTATTCATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4330.10277882613762869No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4290.10182936815945197No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4280.10159200366490778No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4280.10159200366490778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)