FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339695
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19370.570217400903752No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14280.4203771029894464No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11650.3429547093716422No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11410.3358895479768616No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11040.3249974241599082No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10500.30910081102165177No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9040.26612107920340305No Hit
ATTCTATGTTGGGGTGAATTGATGAATCTAGCCACCTGGGTGGGAAGTAA8700.2561121005607972No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8460.24904693916601656No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA8380.24669188536775638No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8250.24286492294558357No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7910.23285594430297765No Hit
CCCACGTGTCGTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC7660.22549640118341452No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7250.21342675046733098No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7200.21195484184341837No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT7140.2101885514947232No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG6950.20459529872385523No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTCTGACATACT6870.202240244925595No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6830.20106271802646491No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA6750.19870766422820468No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6680.19664699215472706No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6530.19223126628298914No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6290.18516610488820853No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6190.18222228764038328No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT6080.17898408866777551No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG5820.17133016382342986No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5790.17044701864908227No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5760.1695638734747347No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5620.16544252932777934No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5620.16544252932777934No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT5490.16161556690560652No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC5470.16102680345604145No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5370.1580829862082162No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5300.15602231413473852No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA5190.15278411516213072No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5090.14984029791430548No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4960.14601333549213263No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4940.1454245720425676No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4890.14395266341865495No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4880.14365828169387243No Hit
CCCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4870.1433638999690899No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT4830.14218637306995982No Hit
GGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4770.14042008272126466No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4770.14042008272126466No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4770.14042008272126466No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4740.1395369375469171No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC4720.13894817409735202No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4670.1374762654734394No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4660.1371818837486569No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4610.13570997512474425No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4610.13570997512474425No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4550.1339436847760491No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4520.13306053960170153No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4500.13247177615213648No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC4430.1304111040786588No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4390.12923357717952869No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC4380.12893919545474616No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTGCCACGCATGCGGCGATGGCC4370.12864481372996364No Hit
GTTTCAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA4320.127172905106051No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG4240.1248178513077908No Hit
CCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAA4210.12393470613344323No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4200.12364032440866071No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4190.12334594268387819No Hit
ATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGAATCTA4110.12099088888561799No Hit
ACATAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG4070.11981336198648787No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4060.11951898026170535No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGA4060.11951898026170535No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3960.1165751630138801No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3930.11569201783953253No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3920.11539763611475001No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA3910.11510325438996746No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACCC3900.11480887266518494No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3880.1142201092156199No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3870.11392572749083738No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC3870.11392572749083738No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3860.11363134576605485No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC3860.11363134576605485No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTCAGTTTCAGTACAATA3850.11333696404127233No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTGGGAT3830.11274820059170726No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3770.11098191024301211No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA3760.11068752851822959No Hit
GGTCTTGCATGGTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGAC3710.10921561989431697No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3690.10862685644475191No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG3690.10862685644475191No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3560.10479989402257907No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG3550.10450551229779655No Hit
GTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGGCTC3520.10362236712344898No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3510.10332798539866646No Hit
ATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG3490.10273922194910139No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3470.10215045849953634No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3440.10126731332518878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)