FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339695
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19810.583170196794183No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14600.42979731818248723No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11130.3276468596829509No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11070.32588056933425574No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10770.31704911759077997No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10270.3023300313516537No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA8980.26435478885470787No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8740.2572896274599273No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8560.25199075641384183No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8320.2449255950190612No Hit
ATTCTATGTTGGGGTGAATTGATGAATCTAGCCACCTGGGTGGGAAGTAA8170.24050986914732334No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7830.23050089050471748No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7740.22785145498167475No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA7540.22196382048602423No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7500.2207862935868941No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7170.21107169666907077No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTCTGACATACT6750.19870766422820468No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT6720.19782451905385715No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6500.19134812110864158No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6360.18722677696168621No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT6190.18222228764038328No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG6120.1801616155669056No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6090.17927847039255804No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6030.17751218004386288No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC5770.16985825519951722No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5720.16838634657560458No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5630.16573691105256186No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5480.16132118518082397No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5380.15837736793299872No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5340.1571998410338686No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5290.155727932409956No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA5250.15455040551082588No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG5170.15219535171256568No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA5150.15160658826300064No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5050.14866277101517536No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5030.14807400756561032No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5020.1477796258408278No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4980.14660209894169768No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC4970.14630771721691516No Hit
GGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4960.14601333549213263No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT4940.1454245720425676No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4830.14218637306995982No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4820.14189199134517727No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4820.14189199134517727No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4800.14130322789561223No Hit
CCCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4610.13570997512474425No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC4600.13541559339996173No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4570.13453244822561414No Hit
CCCACGTGTCGTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4550.1339436847760491No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4530.13335492132648405No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4500.13247177615213648No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4430.1304111040786588No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4360.12835043200518112No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4360.12835043200518112No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4350.1280560502803986No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4300.12658414165648596No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4300.12658414165648596No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4280.12599537820692092No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4270.1257009964821384No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC4200.12364032440866071No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4180.12305156095909567No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4170.12275717923431315No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4130.12157965233518303No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4110.12099088888561799No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG4080.12010774371127039No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTCAGTTTCAGTACAATA4070.11981336198648787No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTGCCACGCATGCGGCGATGGCC4050.11922459853692283No Hit
GTTTCAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA4020.11834145336257526No Hit
ATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGAATCTA4000.11775268991301022No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3980.11716392646344514No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG3960.1165751630138801No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3960.1165751630138801No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3950.11628078128909758No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3920.11539763611475001No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3920.11539763611475001No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3850.11333696404127233No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3840.1130425823164898No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3800.1118650554173597No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3800.1118650554173597No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3740.11009876506866453No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT3730.10980438334388201No Hit
ATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAGACAGGTACAGT3630.10686056609605675No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACCC3620.10656618437127423No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3590.10568303919692666No Hit
TATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA3590.10568303919692666No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC3560.10479989402257907No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGA3540.10421113057301402No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3530.1039167488482315No Hit
TATGCGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCT3510.10332798539866646No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3490.10273922194910139No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3480.10244484022431886No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3480.10244484022431886No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTGGGAT3480.10244484022431886No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTGGGATAGAAC3470.10215045849953634No Hit
CCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAA3460.10185607677475382No Hit
TGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACGTTTCCCTCTTG3410.10038416815084118No Hit
ATGTTAATATGGGCTTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3400.10008978642605866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)