FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332236
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29490.8876220517945075No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA11380.3425276008620378No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11230.33801273793327635No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9680.29135915433607434No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9450.2844363645119734No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAGAGTCCAA8040.2419966529816155No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT7380.22213125609506496No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7040.21189756678987223No Hit
GTTTTAGTACAATGTGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6930.2085866673087805No Hit
TTCTAATACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCGCCTTTC6700.20166387748467957No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGT6410.19293514248907404No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6380.19203216990332173No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA6330.19052721559373456No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6170.18571136180305564No Hit
GTCGTTGACAGTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6160.18541037094113821No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT5670.17066181870718405No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5600.16855488267376204No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAATAAG5370.1616320928496611No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5320.1601271385400739No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACT5280.1589231750924042No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCACTGTCAACGACCGACCT5190.15621425733514732No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5140.15470930302556016No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5060.15230137613022068No Hit
GTATTAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5040.15169939440638583No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4980.14989344923488124No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4900.1474855223395418No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4900.1474855223395418No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4820.14507759544420232No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATG4810.1447766045822849No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4790.14417462285845No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA4770.14357264113461515No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4630.1393587690677711No Hit
GTACGGGACCATGCAAAACCTGCACGATTCCTGCTCAAGGAACCTCTATG4590.13815480562010138No Hit
TTCTTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA4530.1363488604485968No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4460.13424192441517474No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4450.1339409335532573No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4410.1327369701055876No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4410.1327369701055876No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4400.13243597924367015No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4380.1318339975198353No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4340.13063003407216559No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4270.12852309803874354No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC4230.1273191345910738No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4170.1255131894195692No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4160.12521219855765178No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC4150.12491120769573437No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT4140.12461021683381691No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT4120.12400823510998206No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4050.12190129907656004No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4020.12099832649080774No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG3990.12009535390505545No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3830.11527950011437653No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3800.11437652752862423No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATGTGTT3790.1140755366667068No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3770.11347355494287194No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3730.11226959149520221No Hit
ATGTGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3710.11166760977136733No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGT3600.10835671029027559No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3590.10805571942835816No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3560.10715274684260587No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3500.10534680167110126No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT3470.10444382908534897No Hit
GTACAATGTGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3460.10414283822343154No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3460.10414283822343154No Hit
CTATTAAGAATGTCCTCCTGTAAATGAATGTTAGGAAAGGCGGGAGTTTG3440.10354085649959667No Hit
GTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGG3430.10323986563767924No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3400.10233689305192695No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3400.10233689305192695No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3390.10203590219000952No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3380.10173491132809208No Hit
AGTTAAGGGAATAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3360.10113292960425722No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAAGAATGTCCTCCTGT3360.10113292960425722No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT3360.10113292960425722No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC3350.10083193874233978No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3340.10053094788042236No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC3340.10053094788042236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)